Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane

Wolfgang F. Graier, Jolanta Paltauf-Doburzynska, Brent J F Hill, Eleonore Fleischhacker, Bernhard G. Hoebel, Gert M. Kostner, Michael Sturek

Research output: Contribution to journalArticle

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Abstract

1. Endothelial cell activation is correlated with increased cytosolic Ca2+ concentration, often monitored with cytoplasmic Ca2+ dyes, such as fura-2 and Calcium Green-1. We tested the hypothesis that during weak stimulation of porcine artery endothelial cells, focal, subplasmalemmal Ca2+ elevations occur which are controlled by cell membrane Na+-Ca2+ exchange near mitochondrial membrane and superficial endoplasmic reticulum (SER). 2. Bulk Ca2+ concentration ([Ca2+](b)) was monitored using fura-2 or Calcium Green-1 and subplasmalemmal Ca2+ concentration ([Ca2+](sp)) was determined with FFP-18. The distribution of the SER network was estimated using laser scanning and deconvolution microscopy. 3. Sodium fluoride (10 mmol l-1) and submaximal concentrations of bradykinin (Bk; 1. nmol l-1) stimulated Ca2+ entry with no increase in [Ca2+](b). Although inositol 1,4,5-trisphosphate formation and intracellular Ca2+ release in response to both stimuli were similar, Ca2+ entry in response to NaF exceeded that in response to 1 nmol l-1 Bk by fourfold, suggesting additional effects of NaF on Ca2+ entry pathways but stimulation via intracellular Ca2+ release. 4. Prevention of Na2+ exchange activity by decreasing extracellular Na+ unmasked intracellular Ca2+ release in response to NaF and 1 nmol l-1 Bk, indicated by an increase in [Ca2+](b). Thereby, NaF depleted Bk-releasable Ca2+ pools, while mitochondrial Ca2+ content (released with FCCP or oligomycin) and the amount of Ca2+ stored within the cells (released with ionomycin) was increased compared with cells treated with NaF under normal Na+ conditions. The NaF -initiated increase in [Ca2+(b) and depletion of Bk-releasable Ca2+ pool(s) in the low-Na+ condition was diminished by 25 μmol l-1 ryanodine, indicating the involvement of Ca2+- induced Ca2+ release (CICR). 5. In simultaneous recordings of [Ca2+](sp) (with FFP-18) and [Ca2+](b) (with Calcium Green-1), 1 nmol l-1 Bk or 10 mmol l-1 NaF yielded focal [Ca2+] elevation in the subplasmalemmal region with no increase in the perinuclear area. 6. Treatment with 10 μmol l-1 nocodazole caused the SER to collapse and unmasked Ca2+ release in response to 1 nmol l-1 Bk and 10 mmol l-1 NaF, similar to low-Na+ conditions, while the effect of thapsigargin was not changed. 7. These data show that in endothelial cells, focal, subplasmalemmal Ca2+ elevations in response to small or slow IP3 formation occur due to vectorial Ca2+ release from the SER towards the plasmalemma followed by Ca2+ extrusion by Na+-Ca2+ exchange. While these local Ca2+ elevation are not detectable with Ca2+ dyes for the determination of[Ca2+](b), prevention of Ca2+ extrusion or SER disruption yields increases in [Ca2+](b) partially due to CICR. 8. All of the data support our hypothesis that in weakly stimulated endothelial cells, intracellular Ca2+ release and [Ca2+] elevation are limited to the subplasmalemmal region. We propose that the SER co-operates with associated parts of the plasma membrane to control Ca2+ homeostasis, Ca2+ distribution and Ca2+ entry. The existence of such a subplasmalemmal Ca2+ control unit (SCCU) needs to be considered in discussions of Ca2+ signalling, especially when cytoplasmic Ca2+ dyes, such as fura-2 or Calcium Green-1, are used.

Original languageEnglish (US)
Pages (from-to)109-125
Number of pages17
JournalJournal of Physiology
Volume506
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Endoplasmic Reticulum
Swine
Endothelial Cells
Cell Membrane
Fura-2
Coloring Agents
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Nocodazole
Oligomycins
Ryanodine
Sodium Fluoride
Ionomycin
Inositol 1,4,5-Trisphosphate
Thapsigargin
Mitochondrial Membranes
Bradykinin
Confocal Microscopy
Homeostasis
Arteries
calcium green

ASJC Scopus subject areas

  • Physiology

Cite this

Graier, W. F., Paltauf-Doburzynska, J., Hill, B. J. F., Fleischhacker, E., Hoebel, B. G., Kostner, G. M., & Sturek, M. (1998). Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane. Journal of Physiology, 506(1), 109-125. https://doi.org/10.1111/j.1469-7793.1998.109bx.x

Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane. / Graier, Wolfgang F.; Paltauf-Doburzynska, Jolanta; Hill, Brent J F; Fleischhacker, Eleonore; Hoebel, Bernhard G.; Kostner, Gert M.; Sturek, Michael.

In: Journal of Physiology, Vol. 506, No. 1, 01.01.1998, p. 109-125.

Research output: Contribution to journalArticle

Graier, WF, Paltauf-Doburzynska, J, Hill, BJF, Fleischhacker, E, Hoebel, BG, Kostner, GM & Sturek, M 1998, 'Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane', Journal of Physiology, vol. 506, no. 1, pp. 109-125. https://doi.org/10.1111/j.1469-7793.1998.109bx.x
Graier WF, Paltauf-Doburzynska J, Hill BJF, Fleischhacker E, Hoebel BG, Kostner GM et al. Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane. Journal of Physiology. 1998 Jan 1;506(1):109-125. https://doi.org/10.1111/j.1469-7793.1998.109bx.x
Graier, Wolfgang F. ; Paltauf-Doburzynska, Jolanta ; Hill, Brent J F ; Fleischhacker, Eleonore ; Hoebel, Bernhard G. ; Kostner, Gert M. ; Sturek, Michael. / Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane. In: Journal of Physiology. 1998 ; Vol. 506, No. 1. pp. 109-125.
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N2 - 1. Endothelial cell activation is correlated with increased cytosolic Ca2+ concentration, often monitored with cytoplasmic Ca2+ dyes, such as fura-2 and Calcium Green-1. We tested the hypothesis that during weak stimulation of porcine artery endothelial cells, focal, subplasmalemmal Ca2+ elevations occur which are controlled by cell membrane Na+-Ca2+ exchange near mitochondrial membrane and superficial endoplasmic reticulum (SER). 2. Bulk Ca2+ concentration ([Ca2+](b)) was monitored using fura-2 or Calcium Green-1 and subplasmalemmal Ca2+ concentration ([Ca2+](sp)) was determined with FFP-18. The distribution of the SER network was estimated using laser scanning and deconvolution microscopy. 3. Sodium fluoride (10 mmol l-1) and submaximal concentrations of bradykinin (Bk; 1. nmol l-1) stimulated Ca2+ entry with no increase in [Ca2+](b). Although inositol 1,4,5-trisphosphate formation and intracellular Ca2+ release in response to both stimuli were similar, Ca2+ entry in response to NaF exceeded that in response to 1 nmol l-1 Bk by fourfold, suggesting additional effects of NaF on Ca2+ entry pathways but stimulation via intracellular Ca2+ release. 4. Prevention of Na2+ exchange activity by decreasing extracellular Na+ unmasked intracellular Ca2+ release in response to NaF and 1 nmol l-1 Bk, indicated by an increase in [Ca2+](b). Thereby, NaF depleted Bk-releasable Ca2+ pools, while mitochondrial Ca2+ content (released with FCCP or oligomycin) and the amount of Ca2+ stored within the cells (released with ionomycin) was increased compared with cells treated with NaF under normal Na+ conditions. The NaF -initiated increase in [Ca2+(b) and depletion of Bk-releasable Ca2+ pool(s) in the low-Na+ condition was diminished by 25 μmol l-1 ryanodine, indicating the involvement of Ca2+- induced Ca2+ release (CICR). 5. In simultaneous recordings of [Ca2+](sp) (with FFP-18) and [Ca2+](b) (with Calcium Green-1), 1 nmol l-1 Bk or 10 mmol l-1 NaF yielded focal [Ca2+] elevation in the subplasmalemmal region with no increase in the perinuclear area. 6. Treatment with 10 μmol l-1 nocodazole caused the SER to collapse and unmasked Ca2+ release in response to 1 nmol l-1 Bk and 10 mmol l-1 NaF, similar to low-Na+ conditions, while the effect of thapsigargin was not changed. 7. These data show that in endothelial cells, focal, subplasmalemmal Ca2+ elevations in response to small or slow IP3 formation occur due to vectorial Ca2+ release from the SER towards the plasmalemma followed by Ca2+ extrusion by Na+-Ca2+ exchange. While these local Ca2+ elevation are not detectable with Ca2+ dyes for the determination of[Ca2+](b), prevention of Ca2+ extrusion or SER disruption yields increases in [Ca2+](b) partially due to CICR. 8. All of the data support our hypothesis that in weakly stimulated endothelial cells, intracellular Ca2+ release and [Ca2+] elevation are limited to the subplasmalemmal region. We propose that the SER co-operates with associated parts of the plasma membrane to control Ca2+ homeostasis, Ca2+ distribution and Ca2+ entry. The existence of such a subplasmalemmal Ca2+ control unit (SCCU) needs to be considered in discussions of Ca2+ signalling, especially when cytoplasmic Ca2+ dyes, such as fura-2 or Calcium Green-1, are used.

AB - 1. Endothelial cell activation is correlated with increased cytosolic Ca2+ concentration, often monitored with cytoplasmic Ca2+ dyes, such as fura-2 and Calcium Green-1. We tested the hypothesis that during weak stimulation of porcine artery endothelial cells, focal, subplasmalemmal Ca2+ elevations occur which are controlled by cell membrane Na+-Ca2+ exchange near mitochondrial membrane and superficial endoplasmic reticulum (SER). 2. Bulk Ca2+ concentration ([Ca2+](b)) was monitored using fura-2 or Calcium Green-1 and subplasmalemmal Ca2+ concentration ([Ca2+](sp)) was determined with FFP-18. The distribution of the SER network was estimated using laser scanning and deconvolution microscopy. 3. Sodium fluoride (10 mmol l-1) and submaximal concentrations of bradykinin (Bk; 1. nmol l-1) stimulated Ca2+ entry with no increase in [Ca2+](b). Although inositol 1,4,5-trisphosphate formation and intracellular Ca2+ release in response to both stimuli were similar, Ca2+ entry in response to NaF exceeded that in response to 1 nmol l-1 Bk by fourfold, suggesting additional effects of NaF on Ca2+ entry pathways but stimulation via intracellular Ca2+ release. 4. Prevention of Na2+ exchange activity by decreasing extracellular Na+ unmasked intracellular Ca2+ release in response to NaF and 1 nmol l-1 Bk, indicated by an increase in [Ca2+](b). Thereby, NaF depleted Bk-releasable Ca2+ pools, while mitochondrial Ca2+ content (released with FCCP or oligomycin) and the amount of Ca2+ stored within the cells (released with ionomycin) was increased compared with cells treated with NaF under normal Na+ conditions. The NaF -initiated increase in [Ca2+(b) and depletion of Bk-releasable Ca2+ pool(s) in the low-Na+ condition was diminished by 25 μmol l-1 ryanodine, indicating the involvement of Ca2+- induced Ca2+ release (CICR). 5. In simultaneous recordings of [Ca2+](sp) (with FFP-18) and [Ca2+](b) (with Calcium Green-1), 1 nmol l-1 Bk or 10 mmol l-1 NaF yielded focal [Ca2+] elevation in the subplasmalemmal region with no increase in the perinuclear area. 6. Treatment with 10 μmol l-1 nocodazole caused the SER to collapse and unmasked Ca2+ release in response to 1 nmol l-1 Bk and 10 mmol l-1 NaF, similar to low-Na+ conditions, while the effect of thapsigargin was not changed. 7. These data show that in endothelial cells, focal, subplasmalemmal Ca2+ elevations in response to small or slow IP3 formation occur due to vectorial Ca2+ release from the SER towards the plasmalemma followed by Ca2+ extrusion by Na+-Ca2+ exchange. While these local Ca2+ elevation are not detectable with Ca2+ dyes for the determination of[Ca2+](b), prevention of Ca2+ extrusion or SER disruption yields increases in [Ca2+](b) partially due to CICR. 8. All of the data support our hypothesis that in weakly stimulated endothelial cells, intracellular Ca2+ release and [Ca2+] elevation are limited to the subplasmalemmal region. We propose that the SER co-operates with associated parts of the plasma membrane to control Ca2+ homeostasis, Ca2+ distribution and Ca2+ entry. The existence of such a subplasmalemmal Ca2+ control unit (SCCU) needs to be considered in discussions of Ca2+ signalling, especially when cytoplasmic Ca2+ dyes, such as fura-2 or Calcium Green-1, are used.

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