Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane

Wolfgang F. Graier, Jolanta Paltauf-Doburzynska, Brent J.F. Hill, Eleonore Fleischhacker, Bernhard G. Hoebel, Gert M. Kostner, Michael Sturek

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

1. Endothelial cell activation is correlated with increased cytosolic Ca2+ concentration, often monitored with cytoplasmic Ca2+ dyes, such as fura-2 and Calcium Green-1. We tested the hypothesis that during weak stimulation of porcine artery endothelial cells, focal, subplasmalemmal Ca2+ elevations occur which are controlled by cell membrane Na+-Ca2+ exchange near mitochondrial membrane and superficial endoplasmic reticulum (SER). 2. Bulk Ca2+ concentration ([Ca2+](b)) was monitored using fura-2 or Calcium Green-1 and subplasmalemmal Ca2+ concentration ([Ca2+](sp)) was determined with FFP-18. The distribution of the SER network was estimated using laser scanning and deconvolution microscopy. 3. Sodium fluoride (10 mmol l-1) and submaximal concentrations of bradykinin (Bk; 1. nmol l-1) stimulated Ca2+ entry with no increase in [Ca2+](b). Although inositol 1,4,5-trisphosphate formation and intracellular Ca2+ release in response to both stimuli were similar, Ca2+ entry in response to NaF exceeded that in response to 1 nmol l-1 Bk by fourfold, suggesting additional effects of NaF on Ca2+ entry pathways but stimulation via intracellular Ca2+ release. 4. Prevention of Na2+ exchange activity by decreasing extracellular Na+ unmasked intracellular Ca2+ release in response to NaF and 1 nmol l-1 Bk, indicated by an increase in [Ca2+](b). Thereby, NaF depleted Bk-releasable Ca2+ pools, while mitochondrial Ca2+ content (released with FCCP or oligomycin) and the amount of Ca2+ stored within the cells (released with ionomycin) was increased compared with cells treated with NaF under normal Na+ conditions. The NaF -initiated increase in [Ca2+(b) and depletion of Bk-releasable Ca2+ pool(s) in the low-Na+ condition was diminished by 25 μmol l-1 ryanodine, indicating the involvement of Ca2+- induced Ca2+ release (CICR). 5. In simultaneous recordings of [Ca2+](sp) (with FFP-18) and [Ca2+](b) (with Calcium Green-1), 1 nmol l-1 Bk or 10 mmol l-1 NaF yielded focal [Ca2+] elevation in the subplasmalemmal region with no increase in the perinuclear area. 6. Treatment with 10 μmol l-1 nocodazole caused the SER to collapse and unmasked Ca2+ release in response to 1 nmol l-1 Bk and 10 mmol l-1 NaF, similar to low-Na+ conditions, while the effect of thapsigargin was not changed. 7. These data show that in endothelial cells, focal, subplasmalemmal Ca2+ elevations in response to small or slow IP3 formation occur due to vectorial Ca2+ release from the SER towards the plasmalemma followed by Ca2+ extrusion by Na+-Ca2+ exchange. While these local Ca2+ elevation are not detectable with Ca2+ dyes for the determination of[Ca2+](b), prevention of Ca2+ extrusion or SER disruption yields increases in [Ca2+](b) partially due to CICR. 8. All of the data support our hypothesis that in weakly stimulated endothelial cells, intracellular Ca2+ release and [Ca2+] elevation are limited to the subplasmalemmal region. We propose that the SER co-operates with associated parts of the plasma membrane to control Ca2+ homeostasis, Ca2+ distribution and Ca2+ entry. The existence of such a subplasmalemmal Ca2+ control unit (SCCU) needs to be considered in discussions of Ca2+ signalling, especially when cytoplasmic Ca2+ dyes, such as fura-2 or Calcium Green-1, are used.

Original languageEnglish (US)
Pages (from-to)109-125
Number of pages17
JournalJournal of Physiology
Volume506
Issue number1
DOIs
StatePublished - Jan 1 1998

ASJC Scopus subject areas

  • Physiology

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