Submersion organ culture of preglandular fetal lung

James A. McAteer, Thomas J. Cavanagh, Andrew P. Evan

Research output: Contribution to journalArticle

Abstract

This report describes a method for organ culture of the fetal rat lung at the early preglandular phase of development. When the intact fetal lung is cultured fully submerged in medium it develops as an expanded hollow organ. This pattern of in vitro morphogenesis provides a specimen uniquely suited to analysis of the pulmonary epithelium by scanning electron microscopy. In this system alveolar Type II cells and cell types of the distal pulmonary airways undergo differentiation in serum-free media.

Original languageEnglish (US)
Pages (from-to)59-62
Number of pages4
JournalJournal of Tissue Culture Methods
Volume8
Issue number2
DOIs
StatePublished - Jun 1983

Keywords

  • cytodifferentiation
  • fetal rat lung
  • hydrated collagen gel
  • organ culture
  • pulmonary epithelium
  • submersion culture

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

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