Substance p-induced increase in vascular permeability in the rat trachea does not depend on neutrophils or other components of circulating blood

Robert A. Bethel, James J. Brokaw, Timothy W. Evans, Jay A. Nadel, Donald M. Mcdonald

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An intravascular injection of substance P is known to increase vascular permeability in the rat trachea. Electrical stimulation of the cervical vagus nerve produces a similar response, presumably by releasing substance P or other tachykinins from sensory nerve endings. In the present study, we sought to determine whether the increase in vascular permeability induced by intravascular substance P or by vagal stimulation requires the presence of neutrophils or other components of circulating blood. To eliminate circulating blood, we perfused into the aorta of anesthetized rats an oxygenated Krebs-Henseleit solution containing albumin and monastral blue, a colloidal pigment that does not cross normal tracheal blood vessels. We then injected substance P intravas-cularly or electrically stimulated the right cervical vagus nerve. Increases in vascular permeability were quantified by using a microspectophotometer to measure the amount of extravasated monastral blue in tracheal whole-mounts. We found that the elimination of neutrophils and other components of circulating blood did not prevent the increase in tracheal vascular permeability induced by intravascular substance P or by vagal stimulation.

Original languageEnglish (US)
Pages (from-to)769-779
Number of pages11
JournalExperimental Lung Research
Volume14
Issue number6
DOIs
StatePublished - 1988
Externally publishedYes

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Capillary Permeability
Substance P
Trachea
Rats
Neutrophils
Blood
Vagus Nerve
Tachykinins
Blood vessels
Sensory Receptor Cells
Pigments
Electric Stimulation
Blood Vessels
Aorta
Albumins
Injections

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Substance p-induced increase in vascular permeability in the rat trachea does not depend on neutrophils or other components of circulating blood. / Bethel, Robert A.; Brokaw, James J.; Evans, Timothy W.; Nadel, Jay A.; Mcdonald, Donald M.

In: Experimental Lung Research, Vol. 14, No. 6, 1988, p. 769-779.

Research output: Contribution to journalArticle

Bethel, Robert A. ; Brokaw, James J. ; Evans, Timothy W. ; Nadel, Jay A. ; Mcdonald, Donald M. / Substance p-induced increase in vascular permeability in the rat trachea does not depend on neutrophils or other components of circulating blood. In: Experimental Lung Research. 1988 ; Vol. 14, No. 6. pp. 769-779.
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