3H-Azidopine photoaffinity labeling of high molecular weight proteins in chloroquine resistant falciparum malaria

Zuguang Ye, Knox Van Dyke, Terence Spearman, Ahmad R. Safa

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4 Scopus citations

Abstract

Using 3H-azidopine, we have succeeded in labeling proteins from chloroquine resistant (CR) human falciparum malaria parasites in the molecular weight range of 155-170 Kd. Vinblastine does not compete, but azidopine blocks the labeling using 3H-azidopine. Relatively little or no labeling of the 155-170 Kd protein is seen in the chloroquine sensitive strain using 3H-azidopine. Further competition can be seen with nicardipine and reserpine (71%) respectively and verapamil (61%), chloroquine (48%), quinacrine (56%), trifluoperazine (32%) and chlorpromazine (33%). We speculate that this may be the glycoprotein responsible for the resistance to chloroquine in falciparum malaria.

Original languageEnglish (US)
Pages (from-to)809-813
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number2
DOIs
StatePublished - Jul 31 1989

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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