Superoxide, hydrogen peroxide, and oxygen toxicity in two free-living nematode species

Janice Blum, Irwin Fridovich

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Two species of free-living nematodes, Turbatrix aceti and Caenorhabditis elegans, exhibited a marked sensitivity to 3 atm of 100% O2. Environmental changes in pH and temperature, which altered nematode respiration, resulted in alterations in the survival of these organisms under high pO2. Levels of defensive enzymes such as superoxide dismutase, catalase, glutathione peroxidase, and dianisidine peroxidase were measured in the two species. No changes in the level of superoxide dismutase or catalase activity were induced by exposure of the nematodes to high pO2. Manipulation of these two enzymes was however achieved using the inhibitors 3-amino-1,2,4-triazole and diethyldithiocarbamate. 3-Amino-1,2,4-triazole (20 mm) eliminated ≥80% of the catalase activity in vivo and diethyldithiocarbamate (5 mm) decreased the level of CuZn superoxide dismutase by ≥70%. Both of these compounds increased the sensitivity of C. elegans to high pO2 toxicity. Compounds capable of intracellular redox-cycling with O2--production, such as plumbagin, increased CN--resistant respiration in the nematodes and imposed an O2-dependent toxicity. These experiments demonstrate the toxicity of intracellular O2- and H2O2 in nematodes and the importance of superoxide dismutase and catalase in providing a defense against these toxic molecules in vivo.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume222
Issue number1
DOIs
StatePublished - Apr 1 1983
Externally publishedYes

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Superoxides
Catalase
Hydrogen Peroxide
Superoxide Dismutase
Toxicity
Amitrole
Oxygen
Ditiocarb
Caenorhabditis elegans
Rhabditoidea
Respiration
Poisons
Enzymes
Glutathione Peroxidase
Oxidation-Reduction
Molecules
Temperature
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Superoxide, hydrogen peroxide, and oxygen toxicity in two free-living nematode species. / Blum, Janice; Fridovich, Irwin.

In: Archives of Biochemistry and Biophysics, Vol. 222, No. 1, 01.04.1983, p. 35-43.

Research output: Contribution to journalArticle

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