Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein

Kehu Yuan, Ling Yi, Jian Chen, Xiuxia Qu, Tingting Qing, Xi Rao, Pengfei Jiang, Jianhe Hu, Zikai Xiong, Yuchun Nie, Xuanling Shi, Wei Wang, Chen Ling, Xiaolei Yin, Keqiang Fan, Luhua Lai, Mingxiao Ding, Hongkui Deng

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in the process of virus fusion. Peptides derived from the HR regions of some viruses have been shown to inhibit the entry of these viruses. SARS-CoV was also predicted to have HR1 and HR2 regions in the S2 protein. Based on this prediction, we designed 25 peptides and screened them using a HIV-luc/SARS pseudotyped virus assay. Two peptides, HR1-1 and HR2-18, were identified as potential inhibitors, with EC50 values of 0.14 and 1.19μM, respectively. The inhibitory effects of these peptides were validated by the wild-type SARS-CoV assay. HR1-1 and HR2-18 can serve as functional probes for dissecting the fusion mechanism of SARS-CoV and also provide the potential of further identifying potent inhibitors for SARS-CoV entry.

Original languageEnglish (US)
Pages (from-to)746-752
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume319
Issue number3
DOIs
StatePublished - Jul 2 2004

Keywords

  • Entry inhibitor
  • Peptides
  • Pseudotyped virus
  • SARS-CoV
  • Spike protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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