This study evaluates ileoendorectal pull-through (IEP) with and without an aganglionic colon patch in an experimental rat model for the surgical treatment of total colonic aganglionosis (TCA). Animals were randomly assigned to Group 1 (no patch, No.=6), Group 2 (right colon patch, No.=8), Group 3 (transverse colon patch, No.=7), Group 4 (left colon patch; No.=6), sham operation (SH, No.=6). and unoperated controls (UC, No.=10). Change in percent body weight at 4 weeks after operation was -30.9±3.68% in Group 1, +5.1±1.67% in Group 2, -3.4±3.96% in Group 3, -1.8±4.17% in Group 4, and +12.7±1.54% in SH (Group 1 v other groups: P<.001). Restoration of initial body weight occurred in 100% in Group 2 and SH (8/8 and 6/6, respectively), 50% in Group 4 (3/6), 42.9% in Group 3 (3/7), and 0% in Group 1 (0/6). Transit time (stomach to anus) was significantly shorter in Group 1. All rats in patched groups had a prolonged transit time compared with Group 1. Manometric studies in IEP rats showed favorable anal canal pressures, which were slightly lower than SH and UC. Water and Na+ absorption were significantly greater in patched groups. Rats with a right colon patch (Group 2) showed slightly greater absorption at 4 weeks. These data suggest that an aganglionic colon patch may be an important adjunct in the surgical treatment of TCA.
- Hirschsprung's disease
- ileoendorectal pull-through
- Total colonic aganglionosis
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health