Survival of Schwann cells in chronically denervated skeletal muscles

Eduard I. Dedkov, Tatiana Kostrominova, Andrei B. Borisov, Bruce M. Carlson

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

It is well established that over time Schwann cells disappear from the endoneurial space of the distal stump of a chronically transected sciatic nerve trunk. Nevertheless, the status of the Schwann cells within terminal branches of the transected sciatic nerve remains poorly understood. To elucidate this issue we examined the endoneurial space of the intramuscular nerves in rat hindlimb skeletal muscles, which had been denervated for a 25-month period. Based on specific ultrastructural characteristics, we identified a small population of viable Schwann cells within the intramuscular nerve trunks. The surviving Schwann cells continued to be immunopositive for both S-100 protein and neural cell adhesion molecule. In addition, reverse transcription-polymerase chain reaction and/or Western blot analyses have shown that at least two molecules, brain-derived neurotrophic factor and a non-catalytic truncated form of tyrosine protein kinase receptor B, which could potentially participate in the process of nerve repair, were detectable in chronically denervated skeletal muscle. Our results demonstrate that Schwann cells can survive inside the intramuscular nerve trunks of denervated skeletal muscle for a 25-month period without axonal contact.

Original languageEnglish (US)
Pages (from-to)564-574
Number of pages11
JournalActa Neuropathologica
Volume103
Issue number6
StatePublished - 2002
Externally publishedYes

Fingerprint

Schwann Cells
Skeletal Muscle
Sciatic Nerve
Neural Cell Adhesion Molecules
Proto-Oncogene Proteins c-akt
S100 Proteins
Brain-Derived Neurotrophic Factor
Hindlimb
Protein-Tyrosine Kinases
Reverse Transcription
Western Blotting
Polymerase Chain Reaction
Population

Keywords

  • Axotomy
  • Muscle denervation
  • Neural cell adhesion molecule
  • S-100 protein
  • Schwann cells

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Dedkov, E. I., Kostrominova, T., Borisov, A. B., & Carlson, B. M. (2002). Survival of Schwann cells in chronically denervated skeletal muscles. Acta Neuropathologica, 103(6), 564-574.

Survival of Schwann cells in chronically denervated skeletal muscles. / Dedkov, Eduard I.; Kostrominova, Tatiana; Borisov, Andrei B.; Carlson, Bruce M.

In: Acta Neuropathologica, Vol. 103, No. 6, 2002, p. 564-574.

Research output: Contribution to journalArticle

Dedkov, EI, Kostrominova, T, Borisov, AB & Carlson, BM 2002, 'Survival of Schwann cells in chronically denervated skeletal muscles', Acta Neuropathologica, vol. 103, no. 6, pp. 564-574.
Dedkov, Eduard I. ; Kostrominova, Tatiana ; Borisov, Andrei B. ; Carlson, Bruce M. / Survival of Schwann cells in chronically denervated skeletal muscles. In: Acta Neuropathologica. 2002 ; Vol. 103, No. 6. pp. 564-574.
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