SV40 T antigen and telomerase are required to obtain immortalized human adult bone cells without loss of the differentiated phenotype

Christian Darimont, Ornella Avanti, Yvonne Tromvoukis, Patricia Vautravers-Leone, Noriyoshi Kurihara, G. David Roodman, Lorel M. Colgin, Heide Tullberg-Reinert, Andrea M A Pfeifer, Elizabeth A. Offord, Katherine Mace

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In most human primary bone cells, SV40 T-antigen expression was able to expand life span for a few passages before cells undergo growth arrest, described as crisis. In this study, telomerase activity was reconstituted in human osteoblast precursors (hPOB cells) and marrow stromal cells (Saka cells) transformed with the SV40 T antigen. Bone cells with telomerase activity were able to bypass crisis and show unlimited life span. Despite chromosomal aberrations observed in hPOB-tert cells, these immortalized precursors were able to differentiate into osteoblasts like precrisis hPOB cells. Saka-tert cells enhanced the formation of human osteoclast-like cells in a similar manner as Saka cells. These results demonstrate that reconstitution of telomerase activity in transformed SV40 T-antigen human osteoblast precursors or marrow stromal cells leads to the generation of immortalized cells with a preserved phenotype.

Original languageEnglish (US)
Pages (from-to)59-67
Number of pages9
JournalCell Growth and Differentiation
Volume13
Issue number2
StatePublished - 2002
Externally publishedYes

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Polyomavirus Transforming Antigens
Telomerase
Phenotype
Bone and Bones
Osteoblasts
Stromal Cells
Bone Marrow
Osteoclasts
Chromosome Aberrations

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

SV40 T antigen and telomerase are required to obtain immortalized human adult bone cells without loss of the differentiated phenotype. / Darimont, Christian; Avanti, Ornella; Tromvoukis, Yvonne; Vautravers-Leone, Patricia; Kurihara, Noriyoshi; Roodman, G. David; Colgin, Lorel M.; Tullberg-Reinert, Heide; Pfeifer, Andrea M A; Offord, Elizabeth A.; Mace, Katherine.

In: Cell Growth and Differentiation, Vol. 13, No. 2, 2002, p. 59-67.

Research output: Contribution to journalArticle

Darimont, C, Avanti, O, Tromvoukis, Y, Vautravers-Leone, P, Kurihara, N, Roodman, GD, Colgin, LM, Tullberg-Reinert, H, Pfeifer, AMA, Offord, EA & Mace, K 2002, 'SV40 T antigen and telomerase are required to obtain immortalized human adult bone cells without loss of the differentiated phenotype', Cell Growth and Differentiation, vol. 13, no. 2, pp. 59-67.
Darimont, Christian ; Avanti, Ornella ; Tromvoukis, Yvonne ; Vautravers-Leone, Patricia ; Kurihara, Noriyoshi ; Roodman, G. David ; Colgin, Lorel M. ; Tullberg-Reinert, Heide ; Pfeifer, Andrea M A ; Offord, Elizabeth A. ; Mace, Katherine. / SV40 T antigen and telomerase are required to obtain immortalized human adult bone cells without loss of the differentiated phenotype. In: Cell Growth and Differentiation. 2002 ; Vol. 13, No. 2. pp. 59-67.
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AU - Kurihara, Noriyoshi

AU - Roodman, G. David

AU - Colgin, Lorel M.

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