Sympathetic nerve fibers and ganglia in canine cervical vagus nerves: Localization and quantitation

Patrick Onkka, Waddah Maskoun, Kyoung Suk Rhee, Jessica Hellyer, Jheel Patel, Jian Tan, Lan S. Chen, Harry V. Vinters, Michael C. Fishbein, Peng Sheng Chen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Cervical vagal nerve (CVN) stimulation may improve left ventricular ejection fraction in patients with heart failure. Objectives: To test the hypothesis that sympathetic structures are present in the CVN and to describe the location and quantitate these sympathetic components of the CVN. Methods: We performed immunohistochemical studies of the CVN from 11 normal dogs and simultaneously recorded stellate ganglion nerve activity, left thoracic vagal nerve activity, and subcutaneous electrocardiogram in 2 additional dogs. Results: A total of 28 individual nerve bundles were present in the CVNs of the first 11 dogs, with an average of 1.87±1.06 per dog. All CVNs contain tyrosine hydroxylase-positive (sympathetic) nerves, with a total cross-sectional area of 0.97±0.38 mm2. The sympathetic nerves were nonmyelinated, typically located at the periphery of the nerve bundles and occupied 0.03%-2.80% of the CVN cross-sectional area. Cholineacetyltransferase- positive nerve fibers occupied 12.90%-42.86% of the CVN cross-sectional areas. Ten of 11 CVNs showed tyrosine hydroxylase and cholineacetyltransferase colocalization. In 2 dogs with nerve recordings, we documented heart rate acceleration during spontaneous vagal nerve activity in the absence of stellate ganglion nerve activity. Conclusions: Sympathetic nerve fibers are invariably present in the CVNs of normal dogs and occupy in average up to 2.8% of the cross-sectional area. Because sympathetic nerve fibers are present in the periphery of the CVNs, they may be susceptible to activation by electrical stimulation. Spontaneous activation of the sympathetic component of the vagal nerve may accelerate the heart rate.

Original languageEnglish
Pages (from-to)585-591
Number of pages7
JournalHeart Rhythm
Volume10
Issue number4
DOIs
StatePublished - Apr 2013

Fingerprint

Adrenergic Fibers
Sympathetic Ganglia
Vagus Nerve
Nerve Fibers
Canidae
Dogs
Stellate Ganglion
Tyrosine 3-Monooxygenase
Heart Rate
Thoracic Nerves
Vagus Nerve Stimulation
Stroke Volume
Electric Stimulation
Electrocardiography
Heart Failure

Keywords

  • Cervical vagus nerves
  • Ganglion cells
  • Heart failure
  • Sympathetic nerves
  • Vagal nerve stimulation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Sympathetic nerve fibers and ganglia in canine cervical vagus nerves : Localization and quantitation. / Onkka, Patrick; Maskoun, Waddah; Rhee, Kyoung Suk; Hellyer, Jessica; Patel, Jheel; Tan, Jian; Chen, Lan S.; Vinters, Harry V.; Fishbein, Michael C.; Chen, Peng Sheng.

In: Heart Rhythm, Vol. 10, No. 4, 01.04.2013, p. 585-591.

Research output: Contribution to journalArticle

Onkka, P, Maskoun, W, Rhee, KS, Hellyer, J, Patel, J, Tan, J, Chen, LS, Vinters, HV, Fishbein, MC & Chen, PS 2013, 'Sympathetic nerve fibers and ganglia in canine cervical vagus nerves: Localization and quantitation', Heart Rhythm, vol. 10, no. 4, pp. 585-591. https://doi.org/10.1016/j.hrthm.2012.12.015
Onkka, Patrick ; Maskoun, Waddah ; Rhee, Kyoung Suk ; Hellyer, Jessica ; Patel, Jheel ; Tan, Jian ; Chen, Lan S. ; Vinters, Harry V. ; Fishbein, Michael C. ; Chen, Peng Sheng. / Sympathetic nerve fibers and ganglia in canine cervical vagus nerves : Localization and quantitation. In: Heart Rhythm. 2013 ; Vol. 10, No. 4. pp. 585-591.
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abstract = "Background: Cervical vagal nerve (CVN) stimulation may improve left ventricular ejection fraction in patients with heart failure. Objectives: To test the hypothesis that sympathetic structures are present in the CVN and to describe the location and quantitate these sympathetic components of the CVN. Methods: We performed immunohistochemical studies of the CVN from 11 normal dogs and simultaneously recorded stellate ganglion nerve activity, left thoracic vagal nerve activity, and subcutaneous electrocardiogram in 2 additional dogs. Results: A total of 28 individual nerve bundles were present in the CVNs of the first 11 dogs, with an average of 1.87±1.06 per dog. All CVNs contain tyrosine hydroxylase-positive (sympathetic) nerves, with a total cross-sectional area of 0.97±0.38 mm2. The sympathetic nerves were nonmyelinated, typically located at the periphery of the nerve bundles and occupied 0.03{\%}-2.80{\%} of the CVN cross-sectional area. Cholineacetyltransferase- positive nerve fibers occupied 12.90{\%}-42.86{\%} of the CVN cross-sectional areas. Ten of 11 CVNs showed tyrosine hydroxylase and cholineacetyltransferase colocalization. In 2 dogs with nerve recordings, we documented heart rate acceleration during spontaneous vagal nerve activity in the absence of stellate ganglion nerve activity. Conclusions: Sympathetic nerve fibers are invariably present in the CVNs of normal dogs and occupy in average up to 2.8{\%} of the cross-sectional area. Because sympathetic nerve fibers are present in the periphery of the CVNs, they may be susceptible to activation by electrical stimulation. Spontaneous activation of the sympathetic component of the vagal nerve may accelerate the heart rate.",
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T1 - Sympathetic nerve fibers and ganglia in canine cervical vagus nerves

T2 - Localization and quantitation

AU - Onkka, Patrick

AU - Maskoun, Waddah

AU - Rhee, Kyoung Suk

AU - Hellyer, Jessica

AU - Patel, Jheel

AU - Tan, Jian

AU - Chen, Lan S.

AU - Vinters, Harry V.

AU - Fishbein, Michael C.

AU - Chen, Peng Sheng

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Background: Cervical vagal nerve (CVN) stimulation may improve left ventricular ejection fraction in patients with heart failure. Objectives: To test the hypothesis that sympathetic structures are present in the CVN and to describe the location and quantitate these sympathetic components of the CVN. Methods: We performed immunohistochemical studies of the CVN from 11 normal dogs and simultaneously recorded stellate ganglion nerve activity, left thoracic vagal nerve activity, and subcutaneous electrocardiogram in 2 additional dogs. Results: A total of 28 individual nerve bundles were present in the CVNs of the first 11 dogs, with an average of 1.87±1.06 per dog. All CVNs contain tyrosine hydroxylase-positive (sympathetic) nerves, with a total cross-sectional area of 0.97±0.38 mm2. The sympathetic nerves were nonmyelinated, typically located at the periphery of the nerve bundles and occupied 0.03%-2.80% of the CVN cross-sectional area. Cholineacetyltransferase- positive nerve fibers occupied 12.90%-42.86% of the CVN cross-sectional areas. Ten of 11 CVNs showed tyrosine hydroxylase and cholineacetyltransferase colocalization. In 2 dogs with nerve recordings, we documented heart rate acceleration during spontaneous vagal nerve activity in the absence of stellate ganglion nerve activity. Conclusions: Sympathetic nerve fibers are invariably present in the CVNs of normal dogs and occupy in average up to 2.8% of the cross-sectional area. Because sympathetic nerve fibers are present in the periphery of the CVNs, they may be susceptible to activation by electrical stimulation. Spontaneous activation of the sympathetic component of the vagal nerve may accelerate the heart rate.

AB - Background: Cervical vagal nerve (CVN) stimulation may improve left ventricular ejection fraction in patients with heart failure. Objectives: To test the hypothesis that sympathetic structures are present in the CVN and to describe the location and quantitate these sympathetic components of the CVN. Methods: We performed immunohistochemical studies of the CVN from 11 normal dogs and simultaneously recorded stellate ganglion nerve activity, left thoracic vagal nerve activity, and subcutaneous electrocardiogram in 2 additional dogs. Results: A total of 28 individual nerve bundles were present in the CVNs of the first 11 dogs, with an average of 1.87±1.06 per dog. All CVNs contain tyrosine hydroxylase-positive (sympathetic) nerves, with a total cross-sectional area of 0.97±0.38 mm2. The sympathetic nerves were nonmyelinated, typically located at the periphery of the nerve bundles and occupied 0.03%-2.80% of the CVN cross-sectional area. Cholineacetyltransferase- positive nerve fibers occupied 12.90%-42.86% of the CVN cross-sectional areas. Ten of 11 CVNs showed tyrosine hydroxylase and cholineacetyltransferase colocalization. In 2 dogs with nerve recordings, we documented heart rate acceleration during spontaneous vagal nerve activity in the absence of stellate ganglion nerve activity. Conclusions: Sympathetic nerve fibers are invariably present in the CVNs of normal dogs and occupy in average up to 2.8% of the cross-sectional area. Because sympathetic nerve fibers are present in the periphery of the CVNs, they may be susceptible to activation by electrical stimulation. Spontaneous activation of the sympathetic component of the vagal nerve may accelerate the heart rate.

KW - Cervical vagus nerves

KW - Ganglion cells

KW - Heart failure

KW - Sympathetic nerves

KW - Vagal nerve stimulation

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