Synaptic and membrane properties of neurons in the dorsomedial hypothalamus

Timothy W. Bailey, Grant D. Nicol, John H. Schild, Joseph A. DiMicco

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Studies in intact rats have shown that the dorsomedial hypothalamus (DMH) plays a key role in generating stress-induced physiologic changes, including activation of the hypothalamic-pituitary-adrenal axis through direct projections to paraventricular hypothalamic nucleus (PVN). However, little is known about the cellular properties of DMH neurons. We employed whole-cell patch-clamp recording techniques to characterize membrane properties and spontaneous post-synaptic currents (PSCs) in DMH neurons, including those projecting to PVN (identified by prior injection of DiI into PVN), in rat hypothalamic slices. DMH neurons (n=86 total) had uniform membrane properties. However, PVN-projecting neurons (n=32) had higher action potential (AP) thresholds, and fired fewer APs in response to current injection. Spontaneous PVN-projecting neurons (n=20) also fired APs at lower rates (4.8±0.6 Hz) than spontaneous neurons of unknown projection (n=38; 7.3±1.1 Hz). Spontaneous PSCs were observed in all neurons: One population expressed rapid decay characteristics (1.5-2.0 ms) and was blocked by non-NMDA ionotropic glutamate receptor antagonists NBQX or CNQX. Remaining PSCs reversed near E Cl, were blocked by the GABAA receptor antagonists picrotoxin or bicuculline methiodide (BMI), and had longer decay time constants (4.5-6.0 ms) that were modulated by pentobarbital. Tetrodotoxin markedly reduced the frequency of PSCs sensitive to NBQX but not to BMI. Thus, DMH is made up of electrophysiologically similar neurons and PVN-projecting neurons are less excitable than neurons of unknown projection. Furthermore, as suggested by studies in intact rats, neurons in the DMH, including those projecting to the PVN, are regulated by tonic GABAA and non-NMDA glutamate receptor-mediated synaptic transmission.

Original languageEnglish (US)
Pages (from-to)150-162
Number of pages13
JournalBrain research
Volume985
Issue number2
DOIs
StatePublished - Sep 26 2003

Fingerprint

Synaptic Membranes
Hypothalamus
Paraventricular Hypothalamic Nucleus
Neurons
6-Cyano-7-nitroquinoxaline-2,3-dione
Ionotropic Glutamate Receptors
GABA-A Receptor Antagonists
Picrotoxin
Excitatory Amino Acid Antagonists
Injections
Membranes
Tetrodotoxin
Glutamate Receptors
Patch-Clamp Techniques
Pentobarbital
Synaptic Transmission
Action Potentials

Keywords

  • Dorsomedial hypothalamus
  • GABA receptor
  • Ionotropic glutamate receptor
  • Membrane property
  • Paraventricular hypothalamic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Synaptic and membrane properties of neurons in the dorsomedial hypothalamus. / Bailey, Timothy W.; Nicol, Grant D.; Schild, John H.; DiMicco, Joseph A.

In: Brain research, Vol. 985, No. 2, 26.09.2003, p. 150-162.

Research output: Contribution to journalArticle

Bailey, Timothy W. ; Nicol, Grant D. ; Schild, John H. ; DiMicco, Joseph A. / Synaptic and membrane properties of neurons in the dorsomedial hypothalamus. In: Brain research. 2003 ; Vol. 985, No. 2. pp. 150-162.
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