Synaptosome proteomics.

Fengju Bai, Frank Witzmann

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Our knowledge of the complex synaptic proteome and its relationship to physiological or pathological conditions is rapidly expanding. This has been greatly accelerated by the application of various evolving proteomic techniques, enabling more efficient protein resolution, more accurate protein identification, and more comprehensive characterization of proteins undergoing quantitative and qualitative changes. More recently, the combination of the classical subcellular fractionation techniques for the isolation of synaptosomes from the brain with the various proteomic analyses has facilitated this effort. This has resulted from the enrichment of many low abundant proteins comprising the fundamental structure and molecular machinery of brain neurotransmission and neuroplasticity. The analysis of various subproteomes obtained from the synapse, such as synaptic vesicles, synaptic membranes, presynaptic particles, synaptodendrosomes, and postsynaptic densities (PSD) holds great promise for improving our understanding of the temporal and spatial processes that coordinate synaptic proteins in closely related complexes under both normal and diseased states. This chapter will summarize a selection of recent studies that have drawn upon established and emerging proteomic technologies, along with fractionation techniques that are essential to the isolation and analysis of specific synaptic components, in an effort to understand the complexity and plasticity of the synapse proteome.

Original languageEnglish (US)
Pages (from-to)77-98
Number of pages22
JournalSub-Cellular Biochemistry
Volume43
StatePublished - 2007
Externally publishedYes

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Synaptosomes
Proteomics
Proteome
Fractionation
Proteins
Synapses
Brain
Post-Synaptic Density
Synaptic Membranes
Neuronal Plasticity
Synaptic Vesicles
Molecular Structure
Synaptic Transmission
Machinery
Plasticity
Technology
Membranes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Synaptosome proteomics. / Bai, Fengju; Witzmann, Frank.

In: Sub-Cellular Biochemistry, Vol. 43, 2007, p. 77-98.

Research output: Contribution to journalArticle

Bai, Fengju ; Witzmann, Frank. / Synaptosome proteomics. In: Sub-Cellular Biochemistry. 2007 ; Vol. 43. pp. 77-98.
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