Synergism of Taxol and gallium nitrate in human breast carcinoma cells: Schedule dependency

Yuki Hata, Alan Sandler, Patrick Loehrer, George W. Sledge, George Weber

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Since Taxol (paclitaxel, NSC-125973) arrests cells in mitosis and gallium nitrate (NSC-15200) inhibits cell replication in the S phase, and the two drugs have different biochemical targets, we tested the hypothesis that the combination of these drugs may be synergistic in human breast carcinoma cells. MDAMB-435 human breast carcinoma cells were grown in monolayer in culture. Taxol and gallium inhibited cell proliferation with IC50 = 13 nM and 180 μM, respectively. In clonogenic assays, Taxol and gallium yielded IC50 = 2 nM and 25 μM, respectively. Synergism was observed in both cell proliferation and colony formation assays depending on the treatment schedule. Gallium (300 μM) 24 h prior to Taxol (10 nM) synergistically decreased cell proliferation to 6.5% of untreated cells, which was 50% of the predicted value. In clonogenic assay, gallium (40 μM) given 16 h prior to Taxol (2 nM) yielded 8.7% colony formation of the untreated cells, which was 44% of the predicted value. Combination chemotherapy with Taxol and gallium with this protocol schedule may be useful in the treatment of human breast carcinoma.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalOncology Research
Volume6
Issue number1
StatePublished - 1994

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gallium nitrate
Paclitaxel
Appointments and Schedules
Gallium
Breast Neoplasms
Cell Proliferation
Inhibitory Concentration 50
Drug Combinations
Combination Drug Therapy
S Phase
Mitosis

ASJC Scopus subject areas

  • Cancer Research

Cite this

Synergism of Taxol and gallium nitrate in human breast carcinoma cells : Schedule dependency. / Hata, Yuki; Sandler, Alan; Loehrer, Patrick; Sledge, George W.; Weber, George.

In: Oncology Research, Vol. 6, No. 1, 1994, p. 19-24.

Research output: Contribution to journalArticle

Hata, Y, Sandler, A, Loehrer, P, Sledge, GW & Weber, G 1994, 'Synergism of Taxol and gallium nitrate in human breast carcinoma cells: Schedule dependency', Oncology Research, vol. 6, no. 1, pp. 19-24.
Hata, Yuki ; Sandler, Alan ; Loehrer, Patrick ; Sledge, George W. ; Weber, George. / Synergism of Taxol and gallium nitrate in human breast carcinoma cells : Schedule dependency. In: Oncology Research. 1994 ; Vol. 6, No. 1. pp. 19-24.
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