Synergistic effect of human lactoferrin and recombinant murine interferon-γ on disease progression in mice infected with the polycythemia-inducing strain of the Friend virus complex

L. Lu, R. N. Shen, S. Z. Zhou, C. Srivastava, Maureen Harrington, K. Miyazawa, B. Wu, Z. H. Lin, S. Ruscetti, Hal Broxmeyer

Research output: Contribution to journalArticle

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Abstract

Mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P) have been used as a leukemic mouse model. In the present study, purified iron-saturated human lactoferrin (LF) and recombinant murine (rmu) interferon-γ (IFN-γ), alone or in combination, were used to influence disease progression in virally infected mice. DBA/2 mice were injected i.v. with FVC-P, and were treated s.c. with 100 μg LF at day 7, and/or rmuIFN-γ at 5 x 104 units/day for 3 days beginning at day 6 after viral infection. Mice were assessed for survival, and also 14 days after virus inoculation, the mice were killed and spleen extracts were assessed for spleen focus forming virus (SFFV) titers by spleen focus forming unit (SFFU) assay, SFFV mRNA and genomic DNA expression, and natural killer (NK) cell activity. Treatment with LF or rmuIFN-γ alone had little or no effect on SFFU numbers or SFFV mRNA or genomic DNA expression. However, dramatically decreased SFFV titers and levels of SFFV mRNA and genomic DNA were observed in mice treated with the combination of LF and rmuIFN-γ. NK cell activity decreased by FVC-P was returned to normal levels by LF and rmuIFN-γ. The combined treatment also enhanced the survival rates of FVC-P-infected mice. The results suggest synergistic suppressive effects of LF with rmuIFN-γ on disease progression in FVC-P-infected mice. This information might be of significance as a potential therapy for patients with leukemia and those infected with retroviruses.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalInternational Journal of Hematology
Volume54
Issue number2
StatePublished - 1991

Fingerprint

Friend murine leukemia virus
Polycythemia
Lactoferrin
Spleen Focus-Forming Viruses
Interferons
Disease Progression
Spleen
Viral Load
Natural Killer Cells
Messenger RNA
DNA
Inbred DBA Mouse
Virus Diseases
Retroviridae
Leukemia
Therapeutics
Survival Rate
Iron
Viruses
Survival

ASJC Scopus subject areas

  • Hematology

Cite this

Synergistic effect of human lactoferrin and recombinant murine interferon-γ on disease progression in mice infected with the polycythemia-inducing strain of the Friend virus complex. / Lu, L.; Shen, R. N.; Zhou, S. Z.; Srivastava, C.; Harrington, Maureen; Miyazawa, K.; Wu, B.; Lin, Z. H.; Ruscetti, S.; Broxmeyer, Hal.

In: International Journal of Hematology, Vol. 54, No. 2, 1991, p. 117-124.

Research output: Contribution to journalArticle

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abstract = "Mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P) have been used as a leukemic mouse model. In the present study, purified iron-saturated human lactoferrin (LF) and recombinant murine (rmu) interferon-γ (IFN-γ), alone or in combination, were used to influence disease progression in virally infected mice. DBA/2 mice were injected i.v. with FVC-P, and were treated s.c. with 100 μg LF at day 7, and/or rmuIFN-γ at 5 x 104 units/day for 3 days beginning at day 6 after viral infection. Mice were assessed for survival, and also 14 days after virus inoculation, the mice were killed and spleen extracts were assessed for spleen focus forming virus (SFFV) titers by spleen focus forming unit (SFFU) assay, SFFV mRNA and genomic DNA expression, and natural killer (NK) cell activity. Treatment with LF or rmuIFN-γ alone had little or no effect on SFFU numbers or SFFV mRNA or genomic DNA expression. However, dramatically decreased SFFV titers and levels of SFFV mRNA and genomic DNA were observed in mice treated with the combination of LF and rmuIFN-γ. NK cell activity decreased by FVC-P was returned to normal levels by LF and rmuIFN-γ. The combined treatment also enhanced the survival rates of FVC-P-infected mice. The results suggest synergistic suppressive effects of LF with rmuIFN-γ on disease progression in FVC-P-infected mice. This information might be of significance as a potential therapy for patients with leukemia and those infected with retroviruses.",
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AU - Zhou, S. Z.

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AU - Harrington, Maureen

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