Synergistic effects of purified recombinant human and murine B cell growth factor-1/IL-4 on colony formation in vitro by hematopoietic progenitor cells: Multiple actions

Hal Broxmeyer, L. Lu, S. Cooper, R. Tushinski, D. Mochizuki, B. Y. Rubin, S. Gillis, D. E. Williams

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Abstract

Purified recombinant human B cell growth factor-1/IL-4 was evaluated, alone and in combination, with purified preparations of recombinant human (rhu) CSF or erythropoietin (Epo) for effects on colony formation by human bone marrow CFU-GM progenitor cells (GM) and burst forming unit-E progenitor cells. rhu IL-4 synergized with rhu G-CSF to enhance granulocyte colony formation, but had no effect on CFU-GM colony formation stimulated by rhu GM-CSF, rhu IL-3, or rhu CSF-1. Rhu IL-4 synergized with Epo to enhance BFU-E colony formation equal to that of Epo plus either rhu IL-3, rhu GM-CSF, or rhu G-CSF. Removal of adherent cells and T lymphocytes did not influence the synergistic activities of rhu IL-4. Rmu IL-4, synergized with rhu G-CSF, but not with rmu GM-CSF, rmu IL-3, or natural rmu mu CSF-1, to enhance CFU-GM (mainly granulocyte) colony numvers by a >90% pure preparation of murine CFU-GM. Also, rhu IL-4 at low concentrations enhanced release of CSF and at higher concentrations the release also of suppressor molecules from human monocytes and PHA-stimulated human T lymphocytes. Use of specific CSF antibodies suggested that rhu IL-4 was enhancing the release of G-CSF and CSF-1 from monocytes and the release of GM-CSF and possibly G-CSF from PHA-stimulated T lymphocytes. Use of antibodies for TNF-α, IFN-γ, or TNF-β as well as measurement of TNF and IFN titers suggested that the suppressor molecule(s) released from monocytes were acting with TNF-α and those released from PHA-stimulated T lymphocytes were acting with IFN-γ. These results implicate B cell growth factor-1/IL-4 as a synergistic activity for hematopoietic progenitors and suggest that the actions can be on both progenitor and accessory cells.

Original languageEnglish
Pages (from-to)3852-3862
Number of pages11
JournalJournal of Immunology
Volume141
Issue number11
StatePublished - 1988

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Hematopoietic Stem Cells
Interleukin-4
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Progenitor Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Macrophage Colony-Stimulating Factor
Interleukin-3
Erythropoietin
T-Lymphocytes
Monocytes
Stem Cells
In Vitro Techniques
Granulocytes
Erythroid Precursor Cells
Antibodies
Human Activities

ASJC Scopus subject areas

  • Immunology

Cite this

Synergistic effects of purified recombinant human and murine B cell growth factor-1/IL-4 on colony formation in vitro by hematopoietic progenitor cells : Multiple actions. / Broxmeyer, Hal; Lu, L.; Cooper, S.; Tushinski, R.; Mochizuki, D.; Rubin, B. Y.; Gillis, S.; Williams, D. E.

In: Journal of Immunology, Vol. 141, No. 11, 1988, p. 3852-3862.

Research output: Contribution to journalArticle

Broxmeyer, Hal ; Lu, L. ; Cooper, S. ; Tushinski, R. ; Mochizuki, D. ; Rubin, B. Y. ; Gillis, S. ; Williams, D. E. / Synergistic effects of purified recombinant human and murine B cell growth factor-1/IL-4 on colony formation in vitro by hematopoietic progenitor cells : Multiple actions. In: Journal of Immunology. 1988 ; Vol. 141, No. 11. pp. 3852-3862.
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AU - Broxmeyer, Hal

AU - Lu, L.

AU - Cooper, S.

AU - Tushinski, R.

AU - Mochizuki, D.

AU - Rubin, B. Y.

AU - Gillis, S.

AU - Williams, D. E.

PY - 1988

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