Synergistic inhibition of human marrow granulocyte-macrophage progenitor cells by prostaglandin E and recombinant interferon-α, -β, and -γ and an effect mediated by tumor necrosis factor

Louis Pelus, O. G. Ottmann, K. H. Nocka

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37 Citations (Scopus)

Abstract

The effects of prostaglandin E (PGE) and recombinant human interferon-α, -β, and -γ alone and in combination were tested for their effects on the proliferation of human bone marrow granulocyte-macrophage colony-forming units (GM-CFU). When tested alone, both classes of cytokines inhibited GM-CFU proliferation. In combination, PGE and all three types of recombinant interferons synergized in their ability to inhibit GM-CFU proliferation. Progressive enrichment for marrow GM-CFU indicated that the synergistic effects of PGE and interferon were dependent upon the presence of marrow-adherent cells. Studies using conditioned media from marrow-adherent cells prepared in the presence of interferon-α, -β, and -γ indicated that adherent cells produced a soluble factor in the presence of interferons that subsequently synergized with PGE in inhibiting GM-CFU proliferation. Neutralization of this conditioned media with a monoclonal antibody to tumor necrosis factor abrogated the synergistic inhibition of GM-CFU observed in the presence of PGE. The addition of recombinant tumor necrosis factor and PGE to accessory cell-depleted bone marrow resulted in synergistic inhibition of GM-CFU proliferation.

Original languageEnglish (US)
Pages (from-to)479-484
Number of pages6
JournalJournal of Immunology
Volume140
Issue number2
StatePublished - 1988
Externally publishedYes

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Granulocyte-Macrophage Progenitor Cells
Prostaglandins E
Interferons
Tumor Necrosis Factor-alpha
Bone Marrow
Conditioned Culture Medium
Bone Marrow Cells
Monoclonal Antibodies
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Synergistic inhibition of human marrow granulocyte-macrophage progenitor cells by prostaglandin E and recombinant interferon-α, -β, and -γ and an effect mediated by tumor necrosis factor",
abstract = "The effects of prostaglandin E (PGE) and recombinant human interferon-α, -β, and -γ alone and in combination were tested for their effects on the proliferation of human bone marrow granulocyte-macrophage colony-forming units (GM-CFU). When tested alone, both classes of cytokines inhibited GM-CFU proliferation. In combination, PGE and all three types of recombinant interferons synergized in their ability to inhibit GM-CFU proliferation. Progressive enrichment for marrow GM-CFU indicated that the synergistic effects of PGE and interferon were dependent upon the presence of marrow-adherent cells. Studies using conditioned media from marrow-adherent cells prepared in the presence of interferon-α, -β, and -γ indicated that adherent cells produced a soluble factor in the presence of interferons that subsequently synergized with PGE in inhibiting GM-CFU proliferation. Neutralization of this conditioned media with a monoclonal antibody to tumor necrosis factor abrogated the synergistic inhibition of GM-CFU observed in the presence of PGE. The addition of recombinant tumor necrosis factor and PGE to accessory cell-depleted bone marrow resulted in synergistic inhibition of GM-CFU proliferation.",
author = "Louis Pelus and Ottmann, {O. G.} and Nocka, {K. H.}",
year = "1988",
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T1 - Synergistic inhibition of human marrow granulocyte-macrophage progenitor cells by prostaglandin E and recombinant interferon-α, -β, and -γ and an effect mediated by tumor necrosis factor

AU - Pelus, Louis

AU - Ottmann, O. G.

AU - Nocka, K. H.

PY - 1988

Y1 - 1988

N2 - The effects of prostaglandin E (PGE) and recombinant human interferon-α, -β, and -γ alone and in combination were tested for their effects on the proliferation of human bone marrow granulocyte-macrophage colony-forming units (GM-CFU). When tested alone, both classes of cytokines inhibited GM-CFU proliferation. In combination, PGE and all three types of recombinant interferons synergized in their ability to inhibit GM-CFU proliferation. Progressive enrichment for marrow GM-CFU indicated that the synergistic effects of PGE and interferon were dependent upon the presence of marrow-adherent cells. Studies using conditioned media from marrow-adherent cells prepared in the presence of interferon-α, -β, and -γ indicated that adherent cells produced a soluble factor in the presence of interferons that subsequently synergized with PGE in inhibiting GM-CFU proliferation. Neutralization of this conditioned media with a monoclonal antibody to tumor necrosis factor abrogated the synergistic inhibition of GM-CFU observed in the presence of PGE. The addition of recombinant tumor necrosis factor and PGE to accessory cell-depleted bone marrow resulted in synergistic inhibition of GM-CFU proliferation.

AB - The effects of prostaglandin E (PGE) and recombinant human interferon-α, -β, and -γ alone and in combination were tested for their effects on the proliferation of human bone marrow granulocyte-macrophage colony-forming units (GM-CFU). When tested alone, both classes of cytokines inhibited GM-CFU proliferation. In combination, PGE and all three types of recombinant interferons synergized in their ability to inhibit GM-CFU proliferation. Progressive enrichment for marrow GM-CFU indicated that the synergistic effects of PGE and interferon were dependent upon the presence of marrow-adherent cells. Studies using conditioned media from marrow-adherent cells prepared in the presence of interferon-α, -β, and -γ indicated that adherent cells produced a soluble factor in the presence of interferons that subsequently synergized with PGE in inhibiting GM-CFU proliferation. Neutralization of this conditioned media with a monoclonal antibody to tumor necrosis factor abrogated the synergistic inhibition of GM-CFU observed in the presence of PGE. The addition of recombinant tumor necrosis factor and PGE to accessory cell-depleted bone marrow resulted in synergistic inhibition of GM-CFU proliferation.

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