Syngeneic humoral immune responses to tumor-associated antigens expressed by K-1735 UV-induced melanoma and its metastases

Patricia Thistlethwaithe, Darrell Davidson, Isaiah J. Fidler, Jack A. Roth

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An enzyme-linked immunoassay (ELISA) was developed to study syngeneic humoral immune response to a primary tumor and its metastases in the K-1735 ultraviolet light (UV)-induced C3H murine melanoma system. Binding of sera from syngeneic animals previously immunized with primary tumor or metastatic tumor tissue (M-3, M-4) to corresponding 3 M KCl extracts of tumor was significantly greater than binding of control C3H mouse serum. Antibody binding was not significantly reduced by competitive binding with syngeneic murine muscle or liver extracts, indicating the presence of tumor antigen(s) not shared by normal murine tissue. Antibodies to the tumor-associated antigens were selectively removed by competitive binding with syngeneic K-1735 tumor extracts but not by the unrelated 102 murine sarcoma from C57BL/6. However, EL-4 extracts (C57BL/6) did inhibit antibody binding to the primary and both metastases. Further competitive binding studies demonstrated the presence of a common antigen(s) present on the primary tumor and both metastases. We conclude that the K-1735 UV-induced melanoma primary tumor and its metastases express serologically detectable shared antigenic determinate.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalCancer Immunology Immunotherapy
Volume15
Issue number1
DOIs
StatePublished - Apr 1983
Externally publishedYes

Fingerprint

Neoplasm Antigens
Ultraviolet Rays
Humoral Immunity
Melanoma
Neoplasm Metastasis
Competitive Binding
Neoplasms
Antibodies
Liver Extracts
Inbred C3H Mouse
Serum
Immunoenzyme Techniques
Sarcoma
Antigens
Muscles

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

Cite this

Syngeneic humoral immune responses to tumor-associated antigens expressed by K-1735 UV-induced melanoma and its metastases. / Thistlethwaithe, Patricia; Davidson, Darrell; Fidler, Isaiah J.; Roth, Jack A.

In: Cancer Immunology Immunotherapy, Vol. 15, No. 1, 04.1983, p. 11-16.

Research output: Contribution to journalArticle

Thistlethwaithe, Patricia ; Davidson, Darrell ; Fidler, Isaiah J. ; Roth, Jack A. / Syngeneic humoral immune responses to tumor-associated antigens expressed by K-1735 UV-induced melanoma and its metastases. In: Cancer Immunology Immunotherapy. 1983 ; Vol. 15, No. 1. pp. 11-16.
@article{fbf82c56b5064fb3b907380d7bfa0d40,
title = "Syngeneic humoral immune responses to tumor-associated antigens expressed by K-1735 UV-induced melanoma and its metastases",
abstract = "An enzyme-linked immunoassay (ELISA) was developed to study syngeneic humoral immune response to a primary tumor and its metastases in the K-1735 ultraviolet light (UV)-induced C3H murine melanoma system. Binding of sera from syngeneic animals previously immunized with primary tumor or metastatic tumor tissue (M-3, M-4) to corresponding 3 M KCl extracts of tumor was significantly greater than binding of control C3H mouse serum. Antibody binding was not significantly reduced by competitive binding with syngeneic murine muscle or liver extracts, indicating the presence of tumor antigen(s) not shared by normal murine tissue. Antibodies to the tumor-associated antigens were selectively removed by competitive binding with syngeneic K-1735 tumor extracts but not by the unrelated 102 murine sarcoma from C57BL/6. However, EL-4 extracts (C57BL/6) did inhibit antibody binding to the primary and both metastases. Further competitive binding studies demonstrated the presence of a common antigen(s) present on the primary tumor and both metastases. We conclude that the K-1735 UV-induced melanoma primary tumor and its metastases express serologically detectable shared antigenic determinate.",
author = "Patricia Thistlethwaithe and Darrell Davidson and Fidler, {Isaiah J.} and Roth, {Jack A.}",
year = "1983",
month = "4",
doi = "10.1007/BF00199455",
language = "English (US)",
volume = "15",
pages = "11--16",
journal = "Cancer Immunology, Immunotherapy",
issn = "0340-7004",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "1",

}

TY - JOUR

T1 - Syngeneic humoral immune responses to tumor-associated antigens expressed by K-1735 UV-induced melanoma and its metastases

AU - Thistlethwaithe, Patricia

AU - Davidson, Darrell

AU - Fidler, Isaiah J.

AU - Roth, Jack A.

PY - 1983/4

Y1 - 1983/4

N2 - An enzyme-linked immunoassay (ELISA) was developed to study syngeneic humoral immune response to a primary tumor and its metastases in the K-1735 ultraviolet light (UV)-induced C3H murine melanoma system. Binding of sera from syngeneic animals previously immunized with primary tumor or metastatic tumor tissue (M-3, M-4) to corresponding 3 M KCl extracts of tumor was significantly greater than binding of control C3H mouse serum. Antibody binding was not significantly reduced by competitive binding with syngeneic murine muscle or liver extracts, indicating the presence of tumor antigen(s) not shared by normal murine tissue. Antibodies to the tumor-associated antigens were selectively removed by competitive binding with syngeneic K-1735 tumor extracts but not by the unrelated 102 murine sarcoma from C57BL/6. However, EL-4 extracts (C57BL/6) did inhibit antibody binding to the primary and both metastases. Further competitive binding studies demonstrated the presence of a common antigen(s) present on the primary tumor and both metastases. We conclude that the K-1735 UV-induced melanoma primary tumor and its metastases express serologically detectable shared antigenic determinate.

AB - An enzyme-linked immunoassay (ELISA) was developed to study syngeneic humoral immune response to a primary tumor and its metastases in the K-1735 ultraviolet light (UV)-induced C3H murine melanoma system. Binding of sera from syngeneic animals previously immunized with primary tumor or metastatic tumor tissue (M-3, M-4) to corresponding 3 M KCl extracts of tumor was significantly greater than binding of control C3H mouse serum. Antibody binding was not significantly reduced by competitive binding with syngeneic murine muscle or liver extracts, indicating the presence of tumor antigen(s) not shared by normal murine tissue. Antibodies to the tumor-associated antigens were selectively removed by competitive binding with syngeneic K-1735 tumor extracts but not by the unrelated 102 murine sarcoma from C57BL/6. However, EL-4 extracts (C57BL/6) did inhibit antibody binding to the primary and both metastases. Further competitive binding studies demonstrated the presence of a common antigen(s) present on the primary tumor and both metastases. We conclude that the K-1735 UV-induced melanoma primary tumor and its metastases express serologically detectable shared antigenic determinate.

UR - http://www.scopus.com/inward/record.url?scp=0020638999&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020638999&partnerID=8YFLogxK

U2 - 10.1007/BF00199455

DO - 10.1007/BF00199455

M3 - Article

C2 - 6553502

AN - SCOPUS:0020638999

VL - 15

SP - 11

EP - 16

JO - Cancer Immunology, Immunotherapy

JF - Cancer Immunology, Immunotherapy

SN - 0340-7004

IS - 1

ER -