Synip: A novel insulin-regulated syntaxin 4-binding protein mediating GLUT4 translocation in adipocytes

Jing Min, Shuichi Okada, Makoto Kanzaki, Jeffrey S. Elmendorf, Kenneth J. Coker, Brian P. Ceresa, Li Jyun Syu, Yoichi Noda, Alan R. Saltiel, Jeffrey E. Pessin

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153 Scopus citations

Abstract

Insulin-stimulated glucose transport and GLUT4 translocation require regulated interactions between the v-SNARE, VAMP2, and the t-SNARE, syntaxin 4. We have isolated a novel syntaxin 4-binding protein, Synip, which specifically interacts with syntaxin 4. Insulin induces a dissociation of the Synip:syntaxin 4 complex due to an apparent decrease in the binding affinity of Synip for syntaxin 4. In contrast, the carboxy-terminal domain of Synip does not dissociate from syntaxin 4 in response to insulin stimulation but inhibits glucose transport and GLUT4 translocation. These data implicate Synip as an insulin-regulated syntaxin 4-binding protein directly involved in the control of glucose transport and GLUT4 vesicle translocation.

Original languageEnglish (US)
Pages (from-to)751-760
Number of pages10
JournalMolecular Cell
Volume3
Issue number6
DOIs
StatePublished - Jun 1999

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Min, J., Okada, S., Kanzaki, M., Elmendorf, J. S., Coker, K. J., Ceresa, B. P., Syu, L. J., Noda, Y., Saltiel, A. R., & Pessin, J. E. (1999). Synip: A novel insulin-regulated syntaxin 4-binding protein mediating GLUT4 translocation in adipocytes. Molecular Cell, 3(6), 751-760. https://doi.org/10.1016/S1097-2765(01)80007-1