Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering

Eliseu A. Münchow, Divya Pankajakshan, Maria T P Albuquerque, Krzysztof Kamocki, Evandro Piva, Richard Gregory, Marco C. Bottino

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives: This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Materials and methods: Poly(ε-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. Results: CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0°). Increasing CaO concentrations progressively reduced the mechanical properties (p ≤ 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Conclusions: Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. Clinical significance: CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalClinical Oral Investigations
DOIs
StateAccepted/In press - Nov 27 2015

Fingerprint

Gelatin
Tissue Engineering
Nanoparticles
Bone and Bones
Cell Survival
X-Ray Emission Spectrometry
Osteomyelitis
Transmission Electron Microscopy
Agar
Alkaline Phosphatase
Staphylococcus aureus
Cell Differentiation
Polymers

Keywords

  • Bacteria
  • CaO
  • Electrospinning
  • MC3T3-E1
  • Nanofibers
  • Osteomyelitis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Münchow, E. A., Pankajakshan, D., Albuquerque, M. T. P., Kamocki, K., Piva, E., Gregory, R., & Bottino, M. C. (Accepted/In press). Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering. Clinical Oral Investigations, 1-13. https://doi.org/10.1007/s00784-015-1671-5

Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering. / Münchow, Eliseu A.; Pankajakshan, Divya; Albuquerque, Maria T P; Kamocki, Krzysztof; Piva, Evandro; Gregory, Richard; Bottino, Marco C.

In: Clinical Oral Investigations, 27.11.2015, p. 1-13.

Research output: Contribution to journalArticle

Münchow, Eliseu A. ; Pankajakshan, Divya ; Albuquerque, Maria T P ; Kamocki, Krzysztof ; Piva, Evandro ; Gregory, Richard ; Bottino, Marco C. / Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering. In: Clinical Oral Investigations. 2015 ; pp. 1-13.
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abstract = "Objectives: This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Materials and methods: Poly(ε-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. Results: CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0°). Increasing CaO concentrations progressively reduced the mechanical properties (p ≤ 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Conclusions: Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. Clinical significance: CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.",
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AU - Münchow, Eliseu A.

AU - Pankajakshan, Divya

AU - Albuquerque, Maria T P

AU - Kamocki, Krzysztof

AU - Piva, Evandro

AU - Gregory, Richard

AU - Bottino, Marco C.

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N2 - Objectives: This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Materials and methods: Poly(ε-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. Results: CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0°). Increasing CaO concentrations progressively reduced the mechanical properties (p ≤ 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Conclusions: Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. Clinical significance: CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.

AB - Objectives: This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Materials and methods: Poly(ε-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. Results: CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0°). Increasing CaO concentrations progressively reduced the mechanical properties (p ≤ 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Conclusions: Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. Clinical significance: CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.

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KW - CaO

KW - Electrospinning

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KW - Nanofibers

KW - Osteomyelitis

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