Synthesis of DNA containing the simian virus 40 origin of replication by the combined action of DNA polymerases α and β

S. H. Lee, T. Eki, J. Hurwitz

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109 Scopus citations

Abstract

Proliferating-cell nuclear antigen (PCNA) mediates the replication of simian virus 40 (SV40) DNA by reversing the effects of a protein that inhibits the elongation reaction. Two other protein fractions, activator I and activator II, were also shown to play important roles in this process. We report that activator II isolated from HeLa cell extracts is a PCNA-dependent DNA polymerase δ that is required for efficient replication of DNA containing the SV40 origin of replication. PCNA-dependent DNA polymerase δ on a DNA singly primed ∅X174 single-stranded circular DNA template required PCNA, a complex of the elongation inhibitor and activator I, and the single-stranded DNA-binding protein essential for SV40 DNA replication. DNA polymerase δ, in contrast to DAN polymerase α, hardly used RNA-primed DNA templates. These results indicate that both DNA polymerase α and δ are involved in SV40 DNA replication in vitro and their activity depends on PCNA, the elongation inhibitor, and activator I.

Original languageEnglish (US)
Pages (from-to)7361-7365
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number19
DOIs
StatePublished - Jan 1 1989

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