Synthesis of immunogenic hepatitis A virus particles by recombinant baculoviruses

Elliot Rosen, Jack T. Stapleton, James McLinden

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recombinant baculoviruses were constructed which contained the hepatitis A virus (HAV) open reading frame (ORF) under the control of the polyhedrin promoter. Northern blot analysis with an HAV-specific oligonucleotide probe demonstrated a single transcript large enough to include the HAV ORF in Spodoptera frugiperda cells infected with these recombinants. Immunoblots revealed a 220 kDa protein representing the HAV polyprotein. In addition, proteins which co-migrated with HAV capsid proteins, and several proteins of intermediate size were present, consistent with processing intermediates. HAV antigen was present in cells infected with the recombinant baculoviruses when assessed by solid-phase radioimmunoassay. This HAV antigen had a buoyant density in caesium chloride gradients similar to HAV empty capsids, and elicited HAV neutralizing antibodies in mice.

Original languageEnglish (US)
Pages (from-to)706-712
Number of pages7
JournalVaccine
Volume11
Issue number7
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Hepatitis A virus
Baculoviridae
virion
Virion
synthesis
Hepatitis A Antigens
Open Reading Frames
open reading frames
Hepatitis A Antibodies
Polyproteins
Spodoptera
Proteins
Oligonucleotide Probes
Capsid
Capsid Proteins
cesium
oligonucleotide probes
Neutralizing Antibodies
capsid
proteins

Keywords

  • antigen
  • baculovirus
  • Hepatitis A

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Synthesis of immunogenic hepatitis A virus particles by recombinant baculoviruses. / Rosen, Elliot; Stapleton, Jack T.; McLinden, James.

In: Vaccine, Vol. 11, No. 7, 1993, p. 706-712.

Research output: Contribution to journalArticle

Rosen, Elliot ; Stapleton, Jack T. ; McLinden, James. / Synthesis of immunogenic hepatitis A virus particles by recombinant baculoviruses. In: Vaccine. 1993 ; Vol. 11, No. 7. pp. 706-712.
@article{7049fe595c464ad6949ff4df8a4d9c1a,
title = "Synthesis of immunogenic hepatitis A virus particles by recombinant baculoviruses",
abstract = "Recombinant baculoviruses were constructed which contained the hepatitis A virus (HAV) open reading frame (ORF) under the control of the polyhedrin promoter. Northern blot analysis with an HAV-specific oligonucleotide probe demonstrated a single transcript large enough to include the HAV ORF in Spodoptera frugiperda cells infected with these recombinants. Immunoblots revealed a 220 kDa protein representing the HAV polyprotein. In addition, proteins which co-migrated with HAV capsid proteins, and several proteins of intermediate size were present, consistent with processing intermediates. HAV antigen was present in cells infected with the recombinant baculoviruses when assessed by solid-phase radioimmunoassay. This HAV antigen had a buoyant density in caesium chloride gradients similar to HAV empty capsids, and elicited HAV neutralizing antibodies in mice.",
keywords = "antigen, baculovirus, Hepatitis A",
author = "Elliot Rosen and Stapleton, {Jack T.} and James McLinden",
year = "1993",
doi = "10.1016/0264-410X(93)90253-T",
language = "English (US)",
volume = "11",
pages = "706--712",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "7",

}

TY - JOUR

T1 - Synthesis of immunogenic hepatitis A virus particles by recombinant baculoviruses

AU - Rosen, Elliot

AU - Stapleton, Jack T.

AU - McLinden, James

PY - 1993

Y1 - 1993

N2 - Recombinant baculoviruses were constructed which contained the hepatitis A virus (HAV) open reading frame (ORF) under the control of the polyhedrin promoter. Northern blot analysis with an HAV-specific oligonucleotide probe demonstrated a single transcript large enough to include the HAV ORF in Spodoptera frugiperda cells infected with these recombinants. Immunoblots revealed a 220 kDa protein representing the HAV polyprotein. In addition, proteins which co-migrated with HAV capsid proteins, and several proteins of intermediate size were present, consistent with processing intermediates. HAV antigen was present in cells infected with the recombinant baculoviruses when assessed by solid-phase radioimmunoassay. This HAV antigen had a buoyant density in caesium chloride gradients similar to HAV empty capsids, and elicited HAV neutralizing antibodies in mice.

AB - Recombinant baculoviruses were constructed which contained the hepatitis A virus (HAV) open reading frame (ORF) under the control of the polyhedrin promoter. Northern blot analysis with an HAV-specific oligonucleotide probe demonstrated a single transcript large enough to include the HAV ORF in Spodoptera frugiperda cells infected with these recombinants. Immunoblots revealed a 220 kDa protein representing the HAV polyprotein. In addition, proteins which co-migrated with HAV capsid proteins, and several proteins of intermediate size were present, consistent with processing intermediates. HAV antigen was present in cells infected with the recombinant baculoviruses when assessed by solid-phase radioimmunoassay. This HAV antigen had a buoyant density in caesium chloride gradients similar to HAV empty capsids, and elicited HAV neutralizing antibodies in mice.

KW - antigen

KW - baculovirus

KW - Hepatitis A

UR - http://www.scopus.com/inward/record.url?scp=0027299276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027299276&partnerID=8YFLogxK

U2 - 10.1016/0264-410X(93)90253-T

DO - 10.1016/0264-410X(93)90253-T

M3 - Article

C2 - 8393604

AN - SCOPUS:0027299276

VL - 11

SP - 706

EP - 712

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 7

ER -