Synthesis of leptin in human placenta

Rosa Señarís, Tomás Garcia-Caballero, Xesús Casabiell, Rosalía Gallego, Ramón Castro, Robert Considine, Carlos Dieguez, Felipe F. Casanueva

Research output: Contribution to journalArticle

312 Citations (Scopus)

Abstract

Gender-based differences in serum leptin levels have been reported in umbilical cord blood, and leptin has been detected in human amniotic fluid. In order to understand if leptin may be directly synthesized by human placentae an analysis made up of several steps was performed. First at all RT-PCR analysis from placenta-derived RNA was used to detect human leptin mRNA. The leptin-like immunoradioactivity detected in placentae extracts was identical to human leptin according to the criteria of charge, immunorecognition, SDS-PAGE analysis and blotting, indicating that intact leptin was found and no variants in size, charge or immunoactivity were present in the placentae. Finally an immunohistochemical analysis showed the presence of leptin in the cytoplasm of syncytiotrophoblast cells but not in the core of villi. In conclusion: leptin is synthesized as a single molecular variant identical to human recombinant leptin in human placentae at delivery.

Original languageEnglish (US)
Pages (from-to)4501-4504
Number of pages4
JournalEndocrinology
Volume138
Issue number10
DOIs
StatePublished - 1997
Externally publishedYes

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Leptin
Placenta
Trophoblasts
Amniotic Fluid
Fetal Blood
Polyacrylamide Gel Electrophoresis
Cytoplasm
RNA
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Señarís, R., Garcia-Caballero, T., Casabiell, X., Gallego, R., Castro, R., Considine, R., ... Casanueva, F. F. (1997). Synthesis of leptin in human placenta. Endocrinology, 138(10), 4501-4504. https://doi.org/10.1210/en.138.10.4501

Synthesis of leptin in human placenta. / Señarís, Rosa; Garcia-Caballero, Tomás; Casabiell, Xesús; Gallego, Rosalía; Castro, Ramón; Considine, Robert; Dieguez, Carlos; Casanueva, Felipe F.

In: Endocrinology, Vol. 138, No. 10, 1997, p. 4501-4504.

Research output: Contribution to journalArticle

Señarís, R, Garcia-Caballero, T, Casabiell, X, Gallego, R, Castro, R, Considine, R, Dieguez, C & Casanueva, FF 1997, 'Synthesis of leptin in human placenta', Endocrinology, vol. 138, no. 10, pp. 4501-4504. https://doi.org/10.1210/en.138.10.4501
Señarís R, Garcia-Caballero T, Casabiell X, Gallego R, Castro R, Considine R et al. Synthesis of leptin in human placenta. Endocrinology. 1997;138(10):4501-4504. https://doi.org/10.1210/en.138.10.4501
Señarís, Rosa ; Garcia-Caballero, Tomás ; Casabiell, Xesús ; Gallego, Rosalía ; Castro, Ramón ; Considine, Robert ; Dieguez, Carlos ; Casanueva, Felipe F. / Synthesis of leptin in human placenta. In: Endocrinology. 1997 ; Vol. 138, No. 10. pp. 4501-4504.
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