Systemic and pulmonary immune response to intrabronchial administration of ovalbumin in calves

Harm HogenEsch, Sandra E. Torregrosa, Della Borie, Claire Gaskell, Terry L. Bowersock

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Local immunization of the respiratory tract may be the best way to achieve protection against respiratory pathogens. In order to do so successfully, it is important to fully understand how the immune response to antigen administered via the respiratory route develops. We studied the respiratory and systemic immune response after subcutaneous (SC) and intrabronchial (IB) inoculation of calves with ovalbumin (OVA). Eight calves received two SC inoculations of OVA and eight other calves received two SC and three additional IB inoculations of OVA. The occurrence of OVA-specific antibodies and antibody-secreting cells (ASC) was measured over time using isotype- specific enzyme linked immunosorbent assay (ELISA) and ELISPOT. SC immunization of calves did not result in OVA-specific IgA in bronchoalveolar lavage (BAL) fluid. Subcutaneous printing followed by intrabronchial challenge caused an initial IgG1 response in the bronchoalveolar lavage fluid, followed by a large IgA response. The presence of IgG1-ASCs indicated that the IgG1 was at least partially locally produced. Most of the OVA- specific IgA in the BAL fluid was secreted by pulmonary ASCs as indicated by the large number of IgA-ASCs in BAL samples and the low serum level of OVA- specific IgA. Antigen-specific IgG1 ASCs were detectable among peripheral blood mononuclear cells after culture with OVA.

Original languageEnglish (US)
Pages (from-to)293-302
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume51
Issue number3-4
DOIs
StatePublished - Jun 1 1996
Externally publishedYes

Fingerprint

ovalbumin
Ovalbumin
lungs
immune response
calves
Lung
Immunoglobulin A
Bronchoalveolar Lavage Fluid
Immunoglobulin G
vaccination
Immunization
immunization
antigens
Enzyme-Linked Immunospot Assay
Antibody-Producing Cells
Printing
antibodies
Histocompatibility Antigens Class II
Bronchoalveolar Lavage
mononuclear leukocytes

Keywords

  • Cattle
  • Lung
  • Mucosal immunity
  • Ovalbumin
  • Respiratory immune response

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Systemic and pulmonary immune response to intrabronchial administration of ovalbumin in calves. / HogenEsch, Harm; Torregrosa, Sandra E.; Borie, Della; Gaskell, Claire; Bowersock, Terry L.

In: Veterinary Immunology and Immunopathology, Vol. 51, No. 3-4, 01.06.1996, p. 293-302.

Research output: Contribution to journalArticle

HogenEsch, Harm ; Torregrosa, Sandra E. ; Borie, Della ; Gaskell, Claire ; Bowersock, Terry L. / Systemic and pulmonary immune response to intrabronchial administration of ovalbumin in calves. In: Veterinary Immunology and Immunopathology. 1996 ; Vol. 51, No. 3-4. pp. 293-302.
@article{8823a6464a354126a6294e88c8650dfe,
title = "Systemic and pulmonary immune response to intrabronchial administration of ovalbumin in calves",
abstract = "Local immunization of the respiratory tract may be the best way to achieve protection against respiratory pathogens. In order to do so successfully, it is important to fully understand how the immune response to antigen administered via the respiratory route develops. We studied the respiratory and systemic immune response after subcutaneous (SC) and intrabronchial (IB) inoculation of calves with ovalbumin (OVA). Eight calves received two SC inoculations of OVA and eight other calves received two SC and three additional IB inoculations of OVA. The occurrence of OVA-specific antibodies and antibody-secreting cells (ASC) was measured over time using isotype- specific enzyme linked immunosorbent assay (ELISA) and ELISPOT. SC immunization of calves did not result in OVA-specific IgA in bronchoalveolar lavage (BAL) fluid. Subcutaneous printing followed by intrabronchial challenge caused an initial IgG1 response in the bronchoalveolar lavage fluid, followed by a large IgA response. The presence of IgG1-ASCs indicated that the IgG1 was at least partially locally produced. Most of the OVA- specific IgA in the BAL fluid was secreted by pulmonary ASCs as indicated by the large number of IgA-ASCs in BAL samples and the low serum level of OVA- specific IgA. Antigen-specific IgG1 ASCs were detectable among peripheral blood mononuclear cells after culture with OVA.",
keywords = "Cattle, Lung, Mucosal immunity, Ovalbumin, Respiratory immune response",
author = "Harm HogenEsch and Torregrosa, {Sandra E.} and Della Borie and Claire Gaskell and Bowersock, {Terry L.}",
year = "1996",
month = "6",
day = "1",
doi = "10.1016/0165-2427(95)05501-0",
language = "English (US)",
volume = "51",
pages = "293--302",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Systemic and pulmonary immune response to intrabronchial administration of ovalbumin in calves

AU - HogenEsch, Harm

AU - Torregrosa, Sandra E.

AU - Borie, Della

AU - Gaskell, Claire

AU - Bowersock, Terry L.

PY - 1996/6/1

Y1 - 1996/6/1

N2 - Local immunization of the respiratory tract may be the best way to achieve protection against respiratory pathogens. In order to do so successfully, it is important to fully understand how the immune response to antigen administered via the respiratory route develops. We studied the respiratory and systemic immune response after subcutaneous (SC) and intrabronchial (IB) inoculation of calves with ovalbumin (OVA). Eight calves received two SC inoculations of OVA and eight other calves received two SC and three additional IB inoculations of OVA. The occurrence of OVA-specific antibodies and antibody-secreting cells (ASC) was measured over time using isotype- specific enzyme linked immunosorbent assay (ELISA) and ELISPOT. SC immunization of calves did not result in OVA-specific IgA in bronchoalveolar lavage (BAL) fluid. Subcutaneous printing followed by intrabronchial challenge caused an initial IgG1 response in the bronchoalveolar lavage fluid, followed by a large IgA response. The presence of IgG1-ASCs indicated that the IgG1 was at least partially locally produced. Most of the OVA- specific IgA in the BAL fluid was secreted by pulmonary ASCs as indicated by the large number of IgA-ASCs in BAL samples and the low serum level of OVA- specific IgA. Antigen-specific IgG1 ASCs were detectable among peripheral blood mononuclear cells after culture with OVA.

AB - Local immunization of the respiratory tract may be the best way to achieve protection against respiratory pathogens. In order to do so successfully, it is important to fully understand how the immune response to antigen administered via the respiratory route develops. We studied the respiratory and systemic immune response after subcutaneous (SC) and intrabronchial (IB) inoculation of calves with ovalbumin (OVA). Eight calves received two SC inoculations of OVA and eight other calves received two SC and three additional IB inoculations of OVA. The occurrence of OVA-specific antibodies and antibody-secreting cells (ASC) was measured over time using isotype- specific enzyme linked immunosorbent assay (ELISA) and ELISPOT. SC immunization of calves did not result in OVA-specific IgA in bronchoalveolar lavage (BAL) fluid. Subcutaneous printing followed by intrabronchial challenge caused an initial IgG1 response in the bronchoalveolar lavage fluid, followed by a large IgA response. The presence of IgG1-ASCs indicated that the IgG1 was at least partially locally produced. Most of the OVA- specific IgA in the BAL fluid was secreted by pulmonary ASCs as indicated by the large number of IgA-ASCs in BAL samples and the low serum level of OVA- specific IgA. Antigen-specific IgG1 ASCs were detectable among peripheral blood mononuclear cells after culture with OVA.

KW - Cattle

KW - Lung

KW - Mucosal immunity

KW - Ovalbumin

KW - Respiratory immune response

UR - http://www.scopus.com/inward/record.url?scp=0030001426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030001426&partnerID=8YFLogxK

U2 - 10.1016/0165-2427(95)05501-0

DO - 10.1016/0165-2427(95)05501-0

M3 - Article

VL - 51

SP - 293

EP - 302

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 3-4

ER -