Systemic immunoglobulin (AL) amyloidosis in a cat

Complete primary structure of a feline lambda light chain

Juris J. Liepnieks, Stephen P. DiBartola, Merrill Benson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A tarsal mass removed from a ten year old cat proved to be an extramedullary plasmacytoma largely composed of amyloid. Systemic amyloidosis was indicated when histological examination at necropsy identified amyloid also in the lymph nodes, spleen, and liver. The amyloid subunit protein was isolated by molecular sieve chromatography of reduced and alkylated fibrils extracted from the mass, and its amino acid sequence was determined by sequence analysis of peptides isolated after digestion with trypsin or Staphylococcus protease. The sequence of the C-terminal half of the protein was homologous to the constant region of immunoglobulin lambda light chains of other species establishing the immunoglobulin origin of the amyloid. The N-terminal half of the protein was homologous to the variable region of human lambda light chains and contained several rare residues in the framework regions. To our knowledge, this is the first complete sequence determination of a feline immunoglobulin light chain, and the first complete sequence determination of an amyloid immunoglobulin light chain from a species other than man.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalAmyloid
Volume3
Issue number3
StatePublished - 1996

Fingerprint

Felidae
Amyloidosis
Amyloid
Immunoglobulins
Cats
Immunoglobulin Light Chains
Light
Sequence Analysis
Immunoglobulin lambda-Chains
Amyloidogenic Proteins
Plasmacytoma
Protein Sequence Analysis
Staphylococcus
Trypsin
Gel Chromatography
Digestion
Amino Acid Sequence
Proteins
Peptide Hydrolases
Spleen

Keywords

  • Cat
  • Extramedullary plasmacytoma
  • Feline
  • Immunoglobulin (AL) amyloidosis
  • Lambda light chain

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Systemic immunoglobulin (AL) amyloidosis in a cat : Complete primary structure of a feline lambda light chain. / Liepnieks, Juris J.; DiBartola, Stephen P.; Benson, Merrill.

In: Amyloid, Vol. 3, No. 3, 1996, p. 177-182.

Research output: Contribution to journalArticle

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