Systemic neutrophil response resulting from dental plaque accumulation

Michael J. Kowolik, Sherie A. Dowsett, Jessica Rodriguez, Manuel De La Rosa, George J. Eckert

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: There is considerable current interest in putative relationships between oral and systemic diseases. Since the host response to oral bacteria may be the critical link in this association, our hypothesis was that dental plaque accumulation in healthy subjects would elicit a systemic inflammatory response. Methods: Twenty-three healthy subjects, aged 18 to 25, participated in a 4-phase study. An initial hygiene phase was followed by a 21-day experimental phase (the so-called experimental gingivitis model) in which subjects refrained from all oral hygiene practices, thus permitting the accumulation of bacterial plaque. At days 0, 7, and 21 total and differential peripheral white blood cell (wbc) counts, together with full mouth plaque and gingivitis scores, were recorded. Following a 28-day recovery phase, in which normal oral hygiene practices were resumed, subjects entered the final 21-day control phase which mirrored the experimental phase but with subjects maintaining normal oral hygiene practices. Results: The experimental model performed as anticipated with a correlation between plaque and gingivitis scores of 0.95, also reflecting subject compliance. Total wbc and neutrophil counts increased during the experimental phase. Furthermore, comparison of neutrophil counts between the experimental and control phases demonstrated a significantly higher cell count for the experimental phase on both days 7 and 21 (P = 0.0301 and 0.009, respectively). For total wbc, this was significant on day 21 (P = 0.0262). Conclusion: The results of this study support the hypothesis that the accumulation of dental plaque can result in a measurable systemic inflammatory response, providing further in vivo data to support a mechanistic relationship between oral and systemic pathology.

Original languageEnglish (US)
Pages (from-to)146-151
Number of pages6
JournalJournal of periodontology
Volume72
Issue number2
DOIs
StatePublished - Feb 1 2001

Fingerprint

Dental Plaque
Gingivitis
Oral Hygiene
Neutrophils
Leukocyte Count
Healthy Volunteers
Mouth Diseases
Theoretical Models
Oral Pathology
Hygiene
Mouth
Leukocytes
Cell Count
Bacteria

Keywords

  • Dental models
  • Dental plaque/adverse effects
  • Inflammatory response
  • Leukocytes
  • Neutrophils
  • Systemic diseases

ASJC Scopus subject areas

  • Periodontics

Cite this

Systemic neutrophil response resulting from dental plaque accumulation. / Kowolik, Michael J.; Dowsett, Sherie A.; Rodriguez, Jessica; De La Rosa, Manuel; Eckert, George J.

In: Journal of periodontology, Vol. 72, No. 2, 01.02.2001, p. 146-151.

Research output: Contribution to journalArticle

Kowolik, Michael J. ; Dowsett, Sherie A. ; Rodriguez, Jessica ; De La Rosa, Manuel ; Eckert, George J. / Systemic neutrophil response resulting from dental plaque accumulation. In: Journal of periodontology. 2001 ; Vol. 72, No. 2. pp. 146-151.
@article{5440c4e9119d4fac8cb6449c26034730,
title = "Systemic neutrophil response resulting from dental plaque accumulation",
abstract = "Background: There is considerable current interest in putative relationships between oral and systemic diseases. Since the host response to oral bacteria may be the critical link in this association, our hypothesis was that dental plaque accumulation in healthy subjects would elicit a systemic inflammatory response. Methods: Twenty-three healthy subjects, aged 18 to 25, participated in a 4-phase study. An initial hygiene phase was followed by a 21-day experimental phase (the so-called experimental gingivitis model) in which subjects refrained from all oral hygiene practices, thus permitting the accumulation of bacterial plaque. At days 0, 7, and 21 total and differential peripheral white blood cell (wbc) counts, together with full mouth plaque and gingivitis scores, were recorded. Following a 28-day recovery phase, in which normal oral hygiene practices were resumed, subjects entered the final 21-day control phase which mirrored the experimental phase but with subjects maintaining normal oral hygiene practices. Results: The experimental model performed as anticipated with a correlation between plaque and gingivitis scores of 0.95, also reflecting subject compliance. Total wbc and neutrophil counts increased during the experimental phase. Furthermore, comparison of neutrophil counts between the experimental and control phases demonstrated a significantly higher cell count for the experimental phase on both days 7 and 21 (P = 0.0301 and 0.009, respectively). For total wbc, this was significant on day 21 (P = 0.0262). Conclusion: The results of this study support the hypothesis that the accumulation of dental plaque can result in a measurable systemic inflammatory response, providing further in vivo data to support a mechanistic relationship between oral and systemic pathology.",
keywords = "Dental models, Dental plaque/adverse effects, Inflammatory response, Leukocytes, Neutrophils, Systemic diseases",
author = "Kowolik, {Michael J.} and Dowsett, {Sherie A.} and Jessica Rodriguez and {De La Rosa}, Manuel and Eckert, {George J.}",
year = "2001",
month = "2",
day = "1",
doi = "10.1902/jop.2001.72.2.146",
language = "English (US)",
volume = "72",
pages = "146--151",
journal = "Journal of Periodontology",
issn = "0022-3492",
publisher = "American Academy of Periodontology",
number = "2",

}

TY - JOUR

T1 - Systemic neutrophil response resulting from dental plaque accumulation

AU - Kowolik, Michael J.

AU - Dowsett, Sherie A.

AU - Rodriguez, Jessica

AU - De La Rosa, Manuel

AU - Eckert, George J.

PY - 2001/2/1

Y1 - 2001/2/1

N2 - Background: There is considerable current interest in putative relationships between oral and systemic diseases. Since the host response to oral bacteria may be the critical link in this association, our hypothesis was that dental plaque accumulation in healthy subjects would elicit a systemic inflammatory response. Methods: Twenty-three healthy subjects, aged 18 to 25, participated in a 4-phase study. An initial hygiene phase was followed by a 21-day experimental phase (the so-called experimental gingivitis model) in which subjects refrained from all oral hygiene practices, thus permitting the accumulation of bacterial plaque. At days 0, 7, and 21 total and differential peripheral white blood cell (wbc) counts, together with full mouth plaque and gingivitis scores, were recorded. Following a 28-day recovery phase, in which normal oral hygiene practices were resumed, subjects entered the final 21-day control phase which mirrored the experimental phase but with subjects maintaining normal oral hygiene practices. Results: The experimental model performed as anticipated with a correlation between plaque and gingivitis scores of 0.95, also reflecting subject compliance. Total wbc and neutrophil counts increased during the experimental phase. Furthermore, comparison of neutrophil counts between the experimental and control phases demonstrated a significantly higher cell count for the experimental phase on both days 7 and 21 (P = 0.0301 and 0.009, respectively). For total wbc, this was significant on day 21 (P = 0.0262). Conclusion: The results of this study support the hypothesis that the accumulation of dental plaque can result in a measurable systemic inflammatory response, providing further in vivo data to support a mechanistic relationship between oral and systemic pathology.

AB - Background: There is considerable current interest in putative relationships between oral and systemic diseases. Since the host response to oral bacteria may be the critical link in this association, our hypothesis was that dental plaque accumulation in healthy subjects would elicit a systemic inflammatory response. Methods: Twenty-three healthy subjects, aged 18 to 25, participated in a 4-phase study. An initial hygiene phase was followed by a 21-day experimental phase (the so-called experimental gingivitis model) in which subjects refrained from all oral hygiene practices, thus permitting the accumulation of bacterial plaque. At days 0, 7, and 21 total and differential peripheral white blood cell (wbc) counts, together with full mouth plaque and gingivitis scores, were recorded. Following a 28-day recovery phase, in which normal oral hygiene practices were resumed, subjects entered the final 21-day control phase which mirrored the experimental phase but with subjects maintaining normal oral hygiene practices. Results: The experimental model performed as anticipated with a correlation between plaque and gingivitis scores of 0.95, also reflecting subject compliance. Total wbc and neutrophil counts increased during the experimental phase. Furthermore, comparison of neutrophil counts between the experimental and control phases demonstrated a significantly higher cell count for the experimental phase on both days 7 and 21 (P = 0.0301 and 0.009, respectively). For total wbc, this was significant on day 21 (P = 0.0262). Conclusion: The results of this study support the hypothesis that the accumulation of dental plaque can result in a measurable systemic inflammatory response, providing further in vivo data to support a mechanistic relationship between oral and systemic pathology.

KW - Dental models

KW - Dental plaque/adverse effects

KW - Inflammatory response

KW - Leukocytes

KW - Neutrophils

KW - Systemic diseases

UR - http://www.scopus.com/inward/record.url?scp=0035259222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035259222&partnerID=8YFLogxK

U2 - 10.1902/jop.2001.72.2.146

DO - 10.1902/jop.2001.72.2.146

M3 - Article

C2 - 11288786

AN - SCOPUS:0035259222

VL - 72

SP - 146

EP - 151

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 2

ER -