T-cell growth factor P40 promotes the proliferation of myeloid cell lines and enhances erythroid burst formation by normal murine bone marrow cells in vitro

D. E. Williams, P. J. Morrissey, D. Y. Mochizuki, P. De Vries, D. Anderson, D. Cosman, H. S. Boswell, S. Cooper, K. H. Grabstein, H. E. Broxmeyer

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

T-cell growth factor P40 was examined for possible effects on murine interleukin-3 (IL-3)-dependent myeloid cell lines and freshly isolated murine bone marrow cells. The results show that P40 stimulated the proliferation of some IL-3-dependent myeloid cell lines of both early myeloid and mast cell phenotype and synergized with IL-3. P40 did not promote proliferation of fresh bone marrow cells, bone marrow enriched for early myeloid cells by 5-fluorouracil treatment, or bone marrow derived mast cells as assessed in 3H-TdR incorporation assays. P40 did not influence the growth of murine colony-forming unit granulocyte-macrophage in agar cultures, either alone or in the presence of optimal or sub-optimal concentrations of CSF-1, GM-colony-stimulating factor, or IL-3. P40 did potentiate burst-forming unit-erythroid (BFU-E) formation in the presence of erythropoietin; however, this was dependent on the cell plating density, suggesting an indirect stimulation of BFU-E by P40. The indirect nature of P40 action on BFU-E was further demonstrated in cell seperation experiments and indicated that the effect was mediated by T cells. These data expand the repertoire of cells that P40 influences.

Original languageEnglish (US)
Pages (from-to)906-911
Number of pages6
JournalBlood
Volume76
Issue number5
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Williams, D. E., Morrissey, P. J., Mochizuki, D. Y., De Vries, P., Anderson, D., Cosman, D., Boswell, H. S., Cooper, S., Grabstein, K. H., & Broxmeyer, H. E. (1990). T-cell growth factor P40 promotes the proliferation of myeloid cell lines and enhances erythroid burst formation by normal murine bone marrow cells in vitro. Blood, 76(5), 906-911.