T cell receptor activation of a ribosomal S6 kinase activity

Víctor Calvo, Barbara E. Bierer, Terry Vik

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Stimulation of the T cell receptor-CD3 complex activates multiple signal transduction pathways, including serine/threonine and tyrosine protein kinases. Stimulation of the human T cell line Jurkat via the T cell receptor-CD3 complex with anti-CD3 monoclonal antibody or incubation with the tumor promoter phorbol 12-myristate 13-acetate caused increases in S6 kinase and microtubule-associated protein 2 (MAP) kinase activities. An S6 kinase activity that was able to phosphorylate exogenous 40S ribosomal S6 protein was recovered in immunoprecipitates obtained using a 90-kDa ribosomal S6 kinase-specific antiserum and thus represents activation of a member of the 90-kDa ribosomal S6 kinase family. Stimulation of the S6 kinase activity correlated with an increase in a kinase activity able to phosphorylate exogenous 90-kDa ribosomal S6 kinase (rsk) attributed to a MAP kinase activity. These increases in S6 and MAP kinase activities further correlated with the appearance of a 42-kDa phosphoprotein detected by anti-phosphotyrosine immunoblotting. However, while the tyrosine phosphorylation of the 42-kDa protein and the MAP kinase activity are dependent on protein kinase C activity, residual S6 kinase activity can be detected following protein kinase C depletion and subsequent anti-CD3 stimulation. Thus, T cell activation through the T cell receptor-CD3 complex results in activation of a member of the 90-kDa S6 kinase family which correlates with, but can be independent of, MAP kinase activation.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalEuropean Journal of Immunology
Volume22
Issue number2
StatePublished - Feb 1992

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Ribosomal Protein S6 Kinases
T-Cell Antigen Receptor
Mitogen-Activated Protein Kinase 3
CD3 Antigens
Protein Kinase C
Ribosomal Protein S6
T-Lymphocytes
S 6
Phosphotyrosine
Protein-Serine-Threonine Kinases
Phosphoproteins
Immunoblotting
Carcinogens
Protein-Tyrosine Kinases
Tyrosine
Immune Sera
Signal Transduction
Acetates
Phosphotransferases
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

T cell receptor activation of a ribosomal S6 kinase activity. / Calvo, Víctor; Bierer, Barbara E.; Vik, Terry.

In: European Journal of Immunology, Vol. 22, No. 2, 02.1992, p. 457-462.

Research output: Contribution to journalArticle

Calvo, Víctor ; Bierer, Barbara E. ; Vik, Terry. / T cell receptor activation of a ribosomal S6 kinase activity. In: European Journal of Immunology. 1992 ; Vol. 22, No. 2. pp. 457-462.
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