T cell recognition of QA-1b antigens on cells lacking a functional Tap-2 transporter

Carla Aldrich, Robert Waltrip, Evan Hermel, Michelle Attaya, Kirsten Fischer Lindahl, John J. Monaco, James Forman

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

MHC class la H chains and β2-microglobulin assemble with appropriate peptides to form stable cell surface molecules that serve as targets for Ag-specific CTL. The structural similarities of class la and the less polymorphic Q/T/M (class Ib) molecules suggest that class Ib molecules also play a role in antigen presentation, although the origin of the peptides they present remains mostly unclear. The cell line RMA-S has a defect in class I Ag presentation, presumably due to a mutation in a peptide transporter gene. This defect can be overcome by transfection of RMA-S cells with the Tap-2 gene (formerly Ham2) that encodes an ATP-binding transporter protein. We now show that a substantial portion of alloreactive CTL specific for Qa-1 class Ib molecules recognize Qa-1b on RMA-S cells and thus differ from most class la specific CTL. Those anti-Qa-1b CTL that do not recognize untransfected RMA-S do lyse RMA-S transfected with Tap-2. We also examine the effects of Qdm, a gene that maps to the D region and alters recognition of Qa-1. Qdmk strains lack an epitope(s) recognized by some (Qdm dependent) anti-Qa-1 CTL whereas Qdm+ strains express this epitope. Thus, Qdm-dependent CTL do not recognize Qa-1 on Qdmk targets whereas Qdm-independent CTL recognize Qa-1 epitopes in all strains. Although Qdm-independent CTL varied as to whether they recognized RMA-S vs RMA, all nine Qdm-dependent clones only recognized Qa-1b on RMA and not RMA-S. This result is consistent with Qdm encoding a peptide dependent upon the TAP transporter for cell membrane expression.

Original languageEnglish (US)
Pages (from-to)3773-3777
Number of pages5
JournalJournal of Immunology
Volume149
Issue number12
StatePublished - 1992
Externally publishedYes

Fingerprint

Epitopes
T-Lymphocytes
Antigens
Peptides
Genes
Antigen Presentation
Transfection
Carrier Proteins
Clone Cells
Adenosine Triphosphate
Cell Membrane
Cell Line
Mutation
peptide permease
transporter associated with antigen processing (TAP)

ASJC Scopus subject areas

  • Immunology

Cite this

Aldrich, C., Waltrip, R., Hermel, E., Attaya, M., Lindahl, K. F., Monaco, J. J., & Forman, J. (1992). T cell recognition of QA-1b antigens on cells lacking a functional Tap-2 transporter. Journal of Immunology, 149(12), 3773-3777.

T cell recognition of QA-1b antigens on cells lacking a functional Tap-2 transporter. / Aldrich, Carla; Waltrip, Robert; Hermel, Evan; Attaya, Michelle; Lindahl, Kirsten Fischer; Monaco, John J.; Forman, James.

In: Journal of Immunology, Vol. 149, No. 12, 1992, p. 3773-3777.

Research output: Contribution to journalArticle

Aldrich, C, Waltrip, R, Hermel, E, Attaya, M, Lindahl, KF, Monaco, JJ & Forman, J 1992, 'T cell recognition of QA-1b antigens on cells lacking a functional Tap-2 transporter', Journal of Immunology, vol. 149, no. 12, pp. 3773-3777.
Aldrich C, Waltrip R, Hermel E, Attaya M, Lindahl KF, Monaco JJ et al. T cell recognition of QA-1b antigens on cells lacking a functional Tap-2 transporter. Journal of Immunology. 1992;149(12):3773-3777.
Aldrich, Carla ; Waltrip, Robert ; Hermel, Evan ; Attaya, Michelle ; Lindahl, Kirsten Fischer ; Monaco, John J. ; Forman, James. / T cell recognition of QA-1b antigens on cells lacking a functional Tap-2 transporter. In: Journal of Immunology. 1992 ; Vol. 149, No. 12. pp. 3773-3777.
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