T-lymphopoietic capacity of cord blood-derived CD34+ progenitor cells

J. P. Gardner, M. Rosenzweig, D. F. Marks, D. Harper, K. Gaynor, Robert Fallon, D. A. Wall, R. P. Johnson, D. T. Scadden

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Umbilical cord blood contains an abundance of CD34+ hematopoietic progenitor cells and has been used in transplantation as an alternative to adult bone marrow or mobilized peripheral blood. Although efficient myelomonocytic, erythroid, and B lymphoid differentiation has been shown in highly purified cord blood CD34+ mononuclear cells lacking expression of lineage-specific antigens, generation of functional T cells has not been previously documented. Exploiting two recently developed, complementary thymic stromal monolayer systems, we show here that immature hematopoietic progenitor cells (CD34+lineage-) from human and rhesus monkey cord blood mononuclear cells undergo T lymphopoiesis in a manner that recapitulates T cell ontogeny in vivo. After 2 weeks of proliferation, cultures contained geloid cells and discrete populations of CD4+CD8+ (double-positive) immature T lymphocytes, followed after an additional 2 weeks by the appearance of single-positive CD4+CD8- and CD4-CD8+ T cells that coexpressed CD3. The T lymphoid phenotype was confirmed at the transcriptional level by the presence of the lymphoid-restricted genes RAG-2 and T cell receptor. T cells generated from cord blood progenitors in these systems exhibited immunofunction as assessed by alloreactive responses in mixed lymphocyte reactions. These findings were comparable between human and rhesus progenitor cells and closely resemble previous data using adult bone marrow CD34+ cells in these models. Together, these observations demonstrate that cord blood contains abundant lymphoid progenitors that undergo T lymphopoiesis in vitro, suggesting the full multipotentiality of this stem cell source and its validity in investigating T lymphoid differentiation.

Original languageEnglish (US)
Pages (from-to)991-999
Number of pages9
JournalExperimental Hematology
Volume26
Issue number10
StatePublished - 1998
Externally publishedYes

Fingerprint

Fetal Blood
Stem Cells
T-Lymphocytes
Lymphopoiesis
Hematopoietic Stem Cells
Mixed Lymphocyte Culture Test
Cell Lineage
T-Cell Antigen Receptor
Macaca mulatta
Bone Marrow Cells
Blood Cells
Transplantation
Bone Marrow
Phenotype
Antigens
Population
Genes

Keywords

  • CD34
  • Cord blood
  • Stem cells
  • T lymphopoiesis

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Gardner, J. P., Rosenzweig, M., Marks, D. F., Harper, D., Gaynor, K., Fallon, R., ... Scadden, D. T. (1998). T-lymphopoietic capacity of cord blood-derived CD34+ progenitor cells. Experimental Hematology, 26(10), 991-999.

T-lymphopoietic capacity of cord blood-derived CD34+ progenitor cells. / Gardner, J. P.; Rosenzweig, M.; Marks, D. F.; Harper, D.; Gaynor, K.; Fallon, Robert; Wall, D. A.; Johnson, R. P.; Scadden, D. T.

In: Experimental Hematology, Vol. 26, No. 10, 1998, p. 991-999.

Research output: Contribution to journalArticle

Gardner, JP, Rosenzweig, M, Marks, DF, Harper, D, Gaynor, K, Fallon, R, Wall, DA, Johnson, RP & Scadden, DT 1998, 'T-lymphopoietic capacity of cord blood-derived CD34+ progenitor cells', Experimental Hematology, vol. 26, no. 10, pp. 991-999.
Gardner JP, Rosenzweig M, Marks DF, Harper D, Gaynor K, Fallon R et al. T-lymphopoietic capacity of cord blood-derived CD34+ progenitor cells. Experimental Hematology. 1998;26(10):991-999.
Gardner, J. P. ; Rosenzweig, M. ; Marks, D. F. ; Harper, D. ; Gaynor, K. ; Fallon, Robert ; Wall, D. A. ; Johnson, R. P. ; Scadden, D. T. / T-lymphopoietic capacity of cord blood-derived CD34+ progenitor cells. In: Experimental Hematology. 1998 ; Vol. 26, No. 10. pp. 991-999.
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