TAP-independent, β2-microglobulin-dependent surface expression of functional mouse CD1.1

Randy Brutkiewicz, Jack R. Bennink, Jonathan W. Yewdell, Albert Bendelac

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

CD1 molecules consist of β2-microglobulin (β2m) noncovalently complexed to a non-major histocompatibility complex (MHC)-encoded monomorphic integral protein homologous to MHC class I α chains. Little is known about the requirements for cell surface expression and T cell recognition of CD1. We inserted the mouse CD1.1 gene into vaccinia virus to create a recombinant virus expressing CD1.1 under the control of a viral promoter. Using this recombinant virus to infect normal or mutant cell lines, we found that the expression of molecules reactive with the CD1.1-specific monoclonal antibody 3C11 requires the expression of β2m but was not affected by the absence of the MHC-encoded peptide transporter (TAP). Consistent with these results, IL- 2 production by the mCDL1-specific T cell hybridoma DN32.D3 was induced by thymocytes from normal mice or mice with a homozygous deletion of the TAP1 gene, but not by thymocytes from mice with a homozygous deletion of the β2m gene. These results indicate that expression of functional mCD1.1 occurs in a β2m-dependent, TAP-independent manner.

Original languageEnglish (US)
Pages (from-to)1913-1919
Number of pages7
JournalJournal of Experimental Medicine
Volume182
Issue number6
DOIs
StatePublished - Dec 1 1995
Externally publishedYes

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Major Histocompatibility Complex
Thymocytes
Viruses
T-Lymphocytes
Vaccinia virus
Gene Deletion
Hybridomas
Genes
Interleukin-2
Monoclonal Antibodies
Cell Line
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

TAP-independent, β2-microglobulin-dependent surface expression of functional mouse CD1.1. / Brutkiewicz, Randy; Bennink, Jack R.; Yewdell, Jonathan W.; Bendelac, Albert.

In: Journal of Experimental Medicine, Vol. 182, No. 6, 01.12.1995, p. 1913-1919.

Research output: Contribution to journalArticle

Brutkiewicz, Randy ; Bennink, Jack R. ; Yewdell, Jonathan W. ; Bendelac, Albert. / TAP-independent, β2-microglobulin-dependent surface expression of functional mouse CD1.1. In: Journal of Experimental Medicine. 1995 ; Vol. 182, No. 6. pp. 1913-1919.
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