Tarantula huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain II voltage sensor in the closed configuration

Yucheng Xiao, Jon Paul Bingham, Weiguo Zhu, Edward Moczydlowski, Songping Liang, Theodore Cummins

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Peptide toxins with high affinity, divergent pharmacological functions, and isoform-specific selectivity are powerful tools for investigating the structure-function relationships of voltage-gated sodium channels (VGSCs). Although a number of interesting inhibitors have been reported from tarantula venoms, little is known about the mechanism for their interaction with VGSCs. We show that huwentoxin-IV (HWTX-IV), a 35-residue peptide from tarantula Ornithoctonus huwena venom, preferentially inhibits neuronal VGSC subtypes rNav1.2, rNav1.3, and hNav1.7 compared with muscle subtypes rNav1.4 and hNav1.5. Of the five VGSCs examined, hNav1.7 was most sensitive to HWTX-IV (IC 50 ∼ 26 nM). Following application of 1 μM HWTX-IV, hNav1.7 currents could only be elicited with extreme depolarizations (>+100 mV). Recovery of hNav1.7 channels from HWTX-IV inhibition could be induced by extreme depolarizations or moderate depolarizations lasting several minutes. Site-directed mutagenesis analysis indicated that the toxin docked at neurotoxin receptor site 4 located at the extracellular S3-S4 linker of domain II. Mutations E818Q and D816N in hNav1.7 decreased toxin affinity for hNav1.7 by ∼300-fold, whereas the reverse mutations in rNav1.4 (N655D/Q657E) and the corresponding mutations in hNav1.5 (R812D/S814E) greatly increased the sensitivity of the muscle VGSCs to HWTX-IV. Our data identify a novel mechanism for sodium channel inhibition by tarantula toxins involving binding to neurotoxin receptor site 4. In contrast to scorpion β-toxins that trap the IIS4 voltage sensor in an outward configuration, we propose that HWTX-IV traps the voltage sensor of domain II in the inward, closed configuration.

Original languageEnglish
Pages (from-to)27300-27313
Number of pages14
JournalJournal of Biological Chemistry
Volume283
Issue number40
DOIs
StatePublished - Oct 3 2008

Fingerprint

Voltage-Gated Sodium Channels
Sodium Channels
Depolarization
Sensors
Electric potential
Neurotoxins
Mutation
Muscle
Spider Venoms
Scorpions
Muscles
Mutagenesis
Peptides
Venoms
Site-Directed Mutagenesis
Protein Isoforms
Pharmacology
Recovery

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Tarantula huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain II voltage sensor in the closed configuration. / Xiao, Yucheng; Bingham, Jon Paul; Zhu, Weiguo; Moczydlowski, Edward; Liang, Songping; Cummins, Theodore.

In: Journal of Biological Chemistry, Vol. 283, No. 40, 03.10.2008, p. 27300-27313.

Research output: Contribution to journalArticle

Xiao, Yucheng ; Bingham, Jon Paul ; Zhu, Weiguo ; Moczydlowski, Edward ; Liang, Songping ; Cummins, Theodore. / Tarantula huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain II voltage sensor in the closed configuration. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 40. pp. 27300-27313.
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