Targeted disruption of ROCK1 causes insulin resistance in vivo

Dae Ho Lee, Jianjian Shi, Nam Ho Jeoung, Min Seon Kim, Janice M. Zabolotny, Sam W. Lee, Morris F. White, Lei Wei, Young Bum Kim

Research output: Contribution to journalArticle

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Abstract

Insulin signaling is essential for normal glucose homeostasis. Rho-kinase (ROCK) isoforms have been shown to participate in insulin signaling and glucose metabolism in cultured cell lines. To investigate the physiological role of ROCK1 in the regulation of whole body glucose homeostasis and insulin sensitivity in vivo, we studied mice with global disruption of ROCK1. Here we show that, at 16-18 weeks of age, ROCK1-deficient mice exhibited insulin resistance, as revealed by the failure of blood glucose levels to decrease after insulin injection. However, glucose tolerance was normal in the absence of ROCK1. These effects were independent of changes in adiposity. Interestingly, ROCK1 gene ablation caused a significant increase in glucose-induced insulin secretion, leading to hyperinsulinemia. To determine the mechanism(s) by which deletion of ROCK1 causes insulin resistance, we measured the ability of insulin to activate phosphatidylinositol 3-kinase and multiple distal pathways in skeletal muscle. Insulin-stimulated phosphatidylinositol 3-kinase activity associated with IRS-1 or phospho-tyrosine was also reduced ∼40% without any alteration in tyrosine phosphorylation of insulin receptor in skeletal muscle. Concurrently, serine phosphorylation of IRS-1 at serine 632/635, which is phosphorylated by ROCK in vitro, was also impaired in these mice. Insulin-induced phosphorylation of Akt, AS160, S6K, and S6 was also decreased in skeletal muscle. These data suggest that ROCK1 deficiency causes systemic insulin resistance by impairing insulin signaling in skeletal muscle. Thus, our results identify ROCK1 as a novel regulator of glucose homeostasis and insulin sensitivity in vivo, which could lead to new treatment approaches for obesity and type 2 diabetes.

Original languageEnglish
Pages (from-to)11776-11780
Number of pages5
JournalJournal of Biological Chemistry
Volume284
Issue number18
DOIs
StatePublished - May 1 2009

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Insulin Resistance
Insulin
Glucose
Skeletal Muscle
Phosphatidylinositol 3-Kinase
Homeostasis
Phosphorylation
Muscle
Serine
Tyrosine
S 6
rho-Associated Kinases
Insulin Receptor
Adiposity
Hyperinsulinism
Type 2 Diabetes Mellitus
Blood Glucose
Cultured Cells
Protein Isoforms
Obesity

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Lee, D. H., Shi, J., Jeoung, N. H., Kim, M. S., Zabolotny, J. M., Lee, S. W., ... Kim, Y. B. (2009). Targeted disruption of ROCK1 causes insulin resistance in vivo. Journal of Biological Chemistry, 284(18), 11776-11780. https://doi.org/10.1074/jbc.C900014200

Targeted disruption of ROCK1 causes insulin resistance in vivo. / Lee, Dae Ho; Shi, Jianjian; Jeoung, Nam Ho; Kim, Min Seon; Zabolotny, Janice M.; Lee, Sam W.; White, Morris F.; Wei, Lei; Kim, Young Bum.

In: Journal of Biological Chemistry, Vol. 284, No. 18, 01.05.2009, p. 11776-11780.

Research output: Contribution to journalArticle

Lee, DH, Shi, J, Jeoung, NH, Kim, MS, Zabolotny, JM, Lee, SW, White, MF, Wei, L & Kim, YB 2009, 'Targeted disruption of ROCK1 causes insulin resistance in vivo', Journal of Biological Chemistry, vol. 284, no. 18, pp. 11776-11780. https://doi.org/10.1074/jbc.C900014200
Lee, Dae Ho ; Shi, Jianjian ; Jeoung, Nam Ho ; Kim, Min Seon ; Zabolotny, Janice M. ; Lee, Sam W. ; White, Morris F. ; Wei, Lei ; Kim, Young Bum. / Targeted disruption of ROCK1 causes insulin resistance in vivo. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 18. pp. 11776-11780.
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