Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities

Scott R. McKercher, Bruce E. Torbett, Karen L. Anderson, Gregory W. Henkel, Deborah J. Vestal, Helene Baribault, Michael Klemsz, Ann J. Feeney, Gillian E. Wu, Christopher J. Paige, Richard A. Maki

Research output: Contribution to journalArticle

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Abstract

PU.1 is a member of the ets family of transcription factors and is expressed exclusively in cells of the hematopoietic lineage. Mice homozygous for a disruption in the PU.1 DNA binding domain are born alive but die of severe septicemia within 48 h. The analysis of these neonates revealed a lack of mature macrophages, neutrophils, B cells and T cells, although erythrocytes and megakaryocytes were present. The absence of lymphoid commitment and development in null mice was not absolute, since mice maintained on antibiotics began to develop normal appearing T cells 3-5 days after birth. In contrast, mature B cells remained undetectable in these older mice. Within the myeloid lineage, despite a lack of macrophages in the older antibiotic-treated animals, a few cells with the characteristics of neutrophils began to appear by day 3. While the PU.1 protein appears not to be essential for myeloid and lymphoid lineage commitment, it is absolutely required for the normal differentiation of B cells and macrophages.

Original languageEnglish
Pages (from-to)5647-5658
Number of pages12
JournalEMBO Journal
Volume15
Issue number20
StatePublished - Oct 15 1996

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Multiple Abnormalities
Macrophages
T-cells
Genes
Cells
B-Lymphocytes
Anti-Bacterial Agents
Neutrophils
Proto-Oncogene Proteins c-ets
T-Lymphocytes
Megakaryocytes
Animals
Cell Lineage
Transcription Factors
Sepsis
Erythrocytes
DNA
Parturition
Newborn Infant
Proteins

Keywords

  • ets family
  • Hematopoiesis
  • PU.1
  • Transcription factor

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

McKercher, S. R., Torbett, B. E., Anderson, K. L., Henkel, G. W., Vestal, D. J., Baribault, H., ... Maki, R. A. (1996). Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities. EMBO Journal, 15(20), 5647-5658.

Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities. / McKercher, Scott R.; Torbett, Bruce E.; Anderson, Karen L.; Henkel, Gregory W.; Vestal, Deborah J.; Baribault, Helene; Klemsz, Michael; Feeney, Ann J.; Wu, Gillian E.; Paige, Christopher J.; Maki, Richard A.

In: EMBO Journal, Vol. 15, No. 20, 15.10.1996, p. 5647-5658.

Research output: Contribution to journalArticle

McKercher, SR, Torbett, BE, Anderson, KL, Henkel, GW, Vestal, DJ, Baribault, H, Klemsz, M, Feeney, AJ, Wu, GE, Paige, CJ & Maki, RA 1996, 'Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities', EMBO Journal, vol. 15, no. 20, pp. 5647-5658.
McKercher SR, Torbett BE, Anderson KL, Henkel GW, Vestal DJ, Baribault H et al. Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities. EMBO Journal. 1996 Oct 15;15(20):5647-5658.
McKercher, Scott R. ; Torbett, Bruce E. ; Anderson, Karen L. ; Henkel, Gregory W. ; Vestal, Deborah J. ; Baribault, Helene ; Klemsz, Michael ; Feeney, Ann J. ; Wu, Gillian E. ; Paige, Christopher J. ; Maki, Richard A. / Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities. In: EMBO Journal. 1996 ; Vol. 15, No. 20. pp. 5647-5658.
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