Targeted expression of SV40 large T-antigen to visceral smooth muscle induces proliferation of contractile smooth muscle cells and results in megacolon

B. Herring, April M. Hoggatt, Aiping F. Smith, Patricia Gallagher

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Many pathological conditions result from the proliferation and de- differentiation of smooth muscle cells leading to impaired contractility of the muscle. Here we show that targeted expression of SV40 large T-antigen to visceral smooth muscle cells in vivo results in increased smooth muscle cell proliferation without de-differentiation or decreased contractility. These data suggest that the de-differentiation and proliferation of smooth muscle cells, seen in many pathological states, may be independently regulated. In the T-antigen transgenic mice the increased smooth muscle cell proliferation results in thickening of the distal colon. Consequently the distal colon becomes hyper-contractile and impedes the flow of digesta through the colon resulting in enlargement of the colon oral to the obstruction. These transgenic mice thus represent a novel model of megacolon that results from increased smooth muscle cell proliferation rather than altered neuronal innervation.

Original languageEnglish
Pages (from-to)17725-17732
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number25
DOIs
StatePublished - Jun 18 1999

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Megacolon
Polyomavirus Transforming Antigens
Viral Tumor Antigens
Smooth Muscle Myocytes
Smooth Muscle
Muscle
Cells
Colon
Cell proliferation
Cell Proliferation
Transgenic Mice
Muscles

ASJC Scopus subject areas

  • Biochemistry

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Targeted expression of SV40 large T-antigen to visceral smooth muscle induces proliferation of contractile smooth muscle cells and results in megacolon. / Herring, B.; Hoggatt, April M.; Smith, Aiping F.; Gallagher, Patricia.

In: Journal of Biological Chemistry, Vol. 274, No. 25, 18.06.1999, p. 17725-17732.

Research output: Contribution to journalArticle

Herring, B. ; Hoggatt, April M. ; Smith, Aiping F. ; Gallagher, Patricia. / Targeted expression of SV40 large T-antigen to visceral smooth muscle induces proliferation of contractile smooth muscle cells and results in megacolon. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 25. pp. 17725-17732.
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