Targeting of tail-anchored membrane proteins to subcellular organelles in Toxoplasma gondii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Proper protein localization is essential for critical cellular processes, including vesicle-mediated transport and protein translocation. Tail-anchored (TA) proteins are integrated into organellar membranes via the C-terminus, orienting the N-terminus towards the cytosol. Localization of TA proteins occurs posttranslationally and is governed by the C-terminus, which contains the integral transmembrane domain (TMD) and targeting sequence. Targeting of TA proteins is dependent on the hydrophobicity of the TMD as well as the length and composition of flanking amino acid sequences. We previously identified an unusual homologue of elongator protein, Elp3, in the apicomplexan parasite Toxoplasma gondii as a TA protein targeting the outer mitochondrial membrane. We sought to gain further insight into TA proteins and their targeting mechanisms using this early-branching eukaryote as a model. Our bioinformatics analysis uncovered 59 predicted TA proteins in Toxoplasma, 9 of which were selected for follow-up analyses based on representative features. We identified novel TA proteins that traffic to specific organelles in Toxoplasma, including the parasite endoplasmic reticulum, mitochondrion, and Golgi apparatus. Domain swap experiments elucidated that targeting of TA proteins to these specific organelles was strongly influenced by the TMD sequence, including charge of the flanking C-terminal sequence.

Original languageEnglish (US)
Pages (from-to)149-158
Number of pages10
JournalTraffic
Volume18
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

Toxoplasma
Organelles
Tail
Membrane Proteins
Proteins
Protein Transport
Parasites
Mitochondrial Membranes
Golgi Apparatus
Membranes
Computational Biology
Eukaryota
Hydrophobic and Hydrophilic Interactions
Mitochondria
Endoplasmic Reticulum
Cytosol
Amino Acid Sequence
Bioinformatics
Hydrophobicity
Carrier Proteins

Keywords

  • endoplasmic reticulum
  • Golgi apparatus
  • membrane protein
  • mitochondria
  • parasites
  • protein localization
  • protein trafficking

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Targeting of tail-anchored membrane proteins to subcellular organelles in Toxoplasma gondii. / Padgett, Leah R.; Arrizabalaga, Gustavo; Sullivan, William.

In: Traffic, Vol. 18, No. 3, 01.03.2017, p. 149-158.

Research output: Contribution to journalArticle

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