Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator

Ian J. Mohr, Robin Clark, Shaw Sun, Elliot Androphy, Paul Macpherson, Michael R. Botchan

Research output: Contribution to journalArticle

300 Citations (Scopus)

Abstract

The mechanism by which transcription factors stimulate DNA replication in eukaryotes is unknown. Bovine papillomavirus DNA synthesis requires the products of the viral E1 gene and the transcriptional activator protein encoded by the E2 gene. Experimental data showed that the 68-kilodalton (kD) E1 protein formed a complex with the 48-kD E2 transcription factor. This complex bound specifically to the viral origin of replication, which contains multiple binding sites for E2. Repressor proteins encoded by the E2 open reading frame failed to complex with E1 suggesting that the 162-amino acid region of E2 that participates in transactivation contained critical determinants for interaction with El. The physical association between a replication protein and a transcription factor suggests that transcriptional activator proteins may function in targeting replication initiator proteins to their respective origins of replication.

Original languageEnglish (US)
Pages (from-to)1694-1699
Number of pages6
JournalScience
Volume250
Issue number4988
StatePublished - Dec 21 1990
Externally publishedYes

Fingerprint

Replication Origin
Trans-Activators
Transcription Factors
Proteins
Repressor Proteins
Viral Proteins
Eukaryota
DNA Replication
Transcriptional Activation
Open Reading Frames
Binding Sites
Amino Acids
DNA
Genes

ASJC Scopus subject areas

  • General

Cite this

Mohr, I. J., Clark, R., Sun, S., Androphy, E., Macpherson, P., & Botchan, M. R. (1990). Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. Science, 250(4988), 1694-1699.

Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. / Mohr, Ian J.; Clark, Robin; Sun, Shaw; Androphy, Elliot; Macpherson, Paul; Botchan, Michael R.

In: Science, Vol. 250, No. 4988, 21.12.1990, p. 1694-1699.

Research output: Contribution to journalArticle

Mohr, IJ, Clark, R, Sun, S, Androphy, E, Macpherson, P & Botchan, MR 1990, 'Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator', Science, vol. 250, no. 4988, pp. 1694-1699.
Mohr, Ian J. ; Clark, Robin ; Sun, Shaw ; Androphy, Elliot ; Macpherson, Paul ; Botchan, Michael R. / Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. In: Science. 1990 ; Vol. 250, No. 4988. pp. 1694-1699.
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