Targeting the LRP5 pathway improves bone properties in a mouse model of osteogenesis imperfecta

Christina M. Jacobsen, Lauren A. Barber, Ugur M. Ayturk, Heather J. Roberts, Lauren E. Deal, Marissa A. Schwartz, Mary Ann Weis, David Eyre, David Zurakowski, Alexander G. Robling, Matthew L. Warman

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

The cell surface receptor low-density lipoprotein receptor-related protein 5 (LRP5) is a key regulator of bone mass and bone strength. Heterozygous missense mutations in LRP5 cause autosomal dominant high bone mass (HBM) in humans by reducing binding to LRP5 by endogenous inhibitors, such as sclerostin (SOST). Mice heterozygous for a knockin allele (Lrp5p.A214V) that is orthologous to a human HBM-causing mutation have increased bone mass and strength. Osteogenesis imperfecta (OI) is a skeletal fragility disorder predominantly caused by mutations that affect type I collagen. We tested whether the LRP5 pathway can be used to improve bone properties in animal models of OI. First, we mated Lrp5+/p.A214Vmice to Col1a2+/p.G610Cmice, which model human type IV OI. We found that Col1a2+/p.G610C;Lrp5+/p.A214Voffspring had significantly increased bone mass and strength compared to Col1a2+/p.G610C; Lrp5+/+littermates. The improved bone properties were not a result of altered mRNA expression of type I collagen or its chaperones, nor were they due to changes in mutant type I collagen secretion. Second, we treated Col1a2+/p.G610Cmice with a monoclonal antibody that inhibits sclerostin activity (Scl-Ab). We found that antibody-treated mice had significantly increased bone mass and strength compared to vehicle-treated littermates. These findings indicate increasing bone formation, even without altering bone collagen composition, may benefit patients with OI.

Original languageEnglish (US)
Pages (from-to)2297-2306
Number of pages10
JournalJournal of Bone and Mineral Research
Volume29
Issue number10
DOIs
StatePublished - Oct 1 2014

Keywords

  • Anabolics
  • Cell/Tissue signaling
  • Diseases and disorders of bone
  • Genetic animal models
  • Osteogenesis imperfecta
  • Paracrine pathways
  • Wnt/Beta-Catenin/LRPS

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

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