Tau gene mutation G389R causes a tauopathy with abundant pick body-like inclusions and axonal deposits

Jill R. Murrell, Maria Grazia Spillantini, Paolo Zolo, Mario Guazzelli, Michael J. Smith, Masato Hasegawa, Francesco Redi, R. Anthony Crowther, Pietro Pietrini, Bernardino Ghetti, Michel Goedert

Research output: Contribution to journalArticle

187 Scopus citations

Abstract

Exonic and intronic mutations in Tau cause familial neurodegenerative syndromes characterized by frontotemporal dementia and dysfunction of multiple cortical and subcortical circuits. Here we describe a G389R mutation in exon 13 of Tau. When 38 years old, the proband presented with progressive aphasia and memory disturbance, followed by apathy, indifference, and hyperphagia. Repeated magnetic resonance imaging showed the dramatic progression of cerebral atrophy. Positron emission tomography revealed marked glucose hypometabolism that was most severe in left frontal, temporal, and parietal cortical regions. Rigidity, pyramidal signs and profound dementia progressed until death at 43 years of age. A paternal uncle, who had died at 43 years of age, had presented with similar symptoms. The proband's brain showed numerous tau-immunoreactive Pick body-like inclusions in the neocortex and the fascia dentata of the hippocampus. In addition, large numbers of tau- positive filamentous inclusions were present in axons in the frontal, temporal, and parietal lobes. Immunoblot analysis of sarkosyl-insoluble tau showed 2 major bands of 60 and 64 kDa. Upon dephosphorylation, these bands resolved into 4 bands consisting of three- and four-repeat tau isoforms. Most isolated tau filaments were straight and resembled filaments found in Alzheimer disease and some frontotemporal dementias with tau mutations. A smaller number of twisted filaments was also observed. Biochemically, recombinant tau proteins with the G389R mutation showed a reduced ability to promote microtubule assembly, suggesting that this may be the primary effect of the mutation. Taken together, the present findings indicate that the G389R mutation in Tau can cause a dementing condition that closely resembles Pick's disease.

Original languageEnglish (US)
Pages (from-to)1207-1226
Number of pages20
JournalJournal of Neuropathology and Experimental Neurology
Volume58
Issue number12
DOIs
StatePublished - Dec 1999

Keywords

  • Axonal tau deposits
  • Brain metabolism
  • Frontotemporal dementia
  • Pick body- like inclusions
  • Tauopathy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

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    Murrell, J. R., Spillantini, M. G., Zolo, P., Guazzelli, M., Smith, M. J., Hasegawa, M., Redi, F., Crowther, R. A., Pietrini, P., Ghetti, B., & Goedert, M. (1999). Tau gene mutation G389R causes a tauopathy with abundant pick body-like inclusions and axonal deposits. Journal of Neuropathology and Experimental Neurology, 58(12), 1207-1226. https://doi.org/10.1097/00005072-199912000-00002