Taurine suppresses the spread of cell death in electrically coupled RPE cells

Chandani Udawatte, Haohua Qian, Nancy J. Mangini, Brian Kennedy, Harris Ripps

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Purpose: To determine whether taurine exerts a protective effect on retinal pigment epithelium (RPE) cells exposed to a cytotoxic agent, cytochrome C (cyC), shown previously to induce apoptosis and produce cell death in electrically coupled neighboring cells. Methods: Monolayer cultures of confluent human RPE (ARPE-19) cells, which express gap-junctional proteins, were incubated in culture medium with or without taurine. After scrape loading cyC into the cells, we assayed these cells for caspase 3 activity and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining to determine the spread of apoptosis. Results: We found that cyC, too large a molecule to traverse gap junctional channels, produced apoptosis in cells injured by the scrape as well as those distant from the site of the scrape, presumably by the intercellular transmission of a toxic agent through the gap junctions that couple these cells. Incubation in taurine, or the gap-junction blocker, octanol, before application of cyC, reduced significantly the fraction of cells undergoing apoptosis. Voltage clamp recordings from electrically coupled Xenopus oocytes transfected with Cx43 showed that junctional communication was unaffected by taurine. Conclusions: Our results indicate that taurine can serve to suppress cell death in RPE cells independent of any effect on gap junctions. We have considered various avenues by which taurine can exert its protective effect, but the precise mechanism involved under these experimental conditions has yet to be identified.

Original languageEnglish (US)
Pages (from-to)1940-1950
Number of pages11
JournalMolecular Vision
Volume14
StatePublished - Oct 29 2008

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Retinal Pigment Epithelium
Taurine
Cell Death
Cytochromes
Gap Junctions
Apoptosis
Octanols
Connexin 43
DNA Nucleotidylexotransferase
Poisons
Cytotoxins
Xenopus
Caspase 3
Oocytes
Culture Media
Communication
Staining and Labeling

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Udawatte, C., Qian, H., Mangini, N. J., Kennedy, B., & Ripps, H. (2008). Taurine suppresses the spread of cell death in electrically coupled RPE cells. Molecular Vision, 14, 1940-1950.

Taurine suppresses the spread of cell death in electrically coupled RPE cells. / Udawatte, Chandani; Qian, Haohua; Mangini, Nancy J.; Kennedy, Brian; Ripps, Harris.

In: Molecular Vision, Vol. 14, 29.10.2008, p. 1940-1950.

Research output: Contribution to journalArticle

Udawatte, C, Qian, H, Mangini, NJ, Kennedy, B & Ripps, H 2008, 'Taurine suppresses the spread of cell death in electrically coupled RPE cells', Molecular Vision, vol. 14, pp. 1940-1950.
Udawatte C, Qian H, Mangini NJ, Kennedy B, Ripps H. Taurine suppresses the spread of cell death in electrically coupled RPE cells. Molecular Vision. 2008 Oct 29;14:1940-1950.
Udawatte, Chandani ; Qian, Haohua ; Mangini, Nancy J. ; Kennedy, Brian ; Ripps, Harris. / Taurine suppresses the spread of cell death in electrically coupled RPE cells. In: Molecular Vision. 2008 ; Vol. 14. pp. 1940-1950.
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