TaxlBPl interacts with papillomavirus E2 and regulates E2-dependent transcription and stability

Xiaoyu Wang, Samisubbu R. Naidu, Francis Sverdrup, Elliot J. Androphy

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The papillomavirus E2 proteins regulate viral replication, gene transcription, and genome maintenance by interacting with other viral and host proteins. From a yeast two-hybrid screen, we identified the cellular protein Tax1BP1 as a novel binding partner of human papillomavirus type 18 (HPV18) E2. Tax1BP1 also interacts with the HPV16 and bovine papillomavirus type 1 (BPV1) E2 proteins, with the C-terminal region of Tax1BP1 interacting with the N-terminal transactivation domain of BPV1 E2. Tax1BP1 complexes with p300 and acts synergistically as a coactivator with p300 to enhance E2-dependent transcription. Using chromatin immuno- precipitation assays, we show that Tax1BP1 and E2 localize to the long control region on the BPV1 genome. Tax1BP1 was recently reported to bind ubiquitin and to function as an essential component of an A20 ubiquitin-editing complex. We demonstrate that Tax1BP1 plays a role in the regulation of the steady-state level of E2 by preventing its proteasomal degradation. These studies provide new insights into the regulation of E2 functions.

Original languageEnglish (US)
Pages (from-to)2274-2284
Number of pages11
JournalJournal of virology
Volume83
Issue number5
DOIs
StatePublished - Mar 1 2009

Fingerprint

Bovine papillomavirus 1
Papillomaviridae
transcription (genetics)
Ubiquitin
ubiquitin
Genome
Human papillomavirus 18
Viral Genes
proteins
Viral Proteins
Protein C
Transcriptional Activation
Chromatin
genome
Proteins
transcriptional activation
virus replication
Yeasts
Maintenance
chromatin

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

TaxlBPl interacts with papillomavirus E2 and regulates E2-dependent transcription and stability. / Wang, Xiaoyu; Naidu, Samisubbu R.; Sverdrup, Francis; Androphy, Elliot J.

In: Journal of virology, Vol. 83, No. 5, 01.03.2009, p. 2274-2284.

Research output: Contribution to journalArticle

Wang, Xiaoyu ; Naidu, Samisubbu R. ; Sverdrup, Francis ; Androphy, Elliot J. / TaxlBPl interacts with papillomavirus E2 and regulates E2-dependent transcription and stability. In: Journal of virology. 2009 ; Vol. 83, No. 5. pp. 2274-2284.
@article{0b21af52b5d747a2bcca498d57b67a59,
title = "TaxlBPl interacts with papillomavirus E2 and regulates E2-dependent transcription and stability",
abstract = "The papillomavirus E2 proteins regulate viral replication, gene transcription, and genome maintenance by interacting with other viral and host proteins. From a yeast two-hybrid screen, we identified the cellular protein Tax1BP1 as a novel binding partner of human papillomavirus type 18 (HPV18) E2. Tax1BP1 also interacts with the HPV16 and bovine papillomavirus type 1 (BPV1) E2 proteins, with the C-terminal region of Tax1BP1 interacting with the N-terminal transactivation domain of BPV1 E2. Tax1BP1 complexes with p300 and acts synergistically as a coactivator with p300 to enhance E2-dependent transcription. Using chromatin immuno- precipitation assays, we show that Tax1BP1 and E2 localize to the long control region on the BPV1 genome. Tax1BP1 was recently reported to bind ubiquitin and to function as an essential component of an A20 ubiquitin-editing complex. We demonstrate that Tax1BP1 plays a role in the regulation of the steady-state level of E2 by preventing its proteasomal degradation. These studies provide new insights into the regulation of E2 functions.",
author = "Xiaoyu Wang and Naidu, {Samisubbu R.} and Francis Sverdrup and Androphy, {Elliot J.}",
year = "2009",
month = "3",
day = "1",
doi = "10.1128/JVI.01791-08",
language = "English (US)",
volume = "83",
pages = "2274--2284",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - TaxlBPl interacts with papillomavirus E2 and regulates E2-dependent transcription and stability

AU - Wang, Xiaoyu

AU - Naidu, Samisubbu R.

AU - Sverdrup, Francis

AU - Androphy, Elliot J.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - The papillomavirus E2 proteins regulate viral replication, gene transcription, and genome maintenance by interacting with other viral and host proteins. From a yeast two-hybrid screen, we identified the cellular protein Tax1BP1 as a novel binding partner of human papillomavirus type 18 (HPV18) E2. Tax1BP1 also interacts with the HPV16 and bovine papillomavirus type 1 (BPV1) E2 proteins, with the C-terminal region of Tax1BP1 interacting with the N-terminal transactivation domain of BPV1 E2. Tax1BP1 complexes with p300 and acts synergistically as a coactivator with p300 to enhance E2-dependent transcription. Using chromatin immuno- precipitation assays, we show that Tax1BP1 and E2 localize to the long control region on the BPV1 genome. Tax1BP1 was recently reported to bind ubiquitin and to function as an essential component of an A20 ubiquitin-editing complex. We demonstrate that Tax1BP1 plays a role in the regulation of the steady-state level of E2 by preventing its proteasomal degradation. These studies provide new insights into the regulation of E2 functions.

AB - The papillomavirus E2 proteins regulate viral replication, gene transcription, and genome maintenance by interacting with other viral and host proteins. From a yeast two-hybrid screen, we identified the cellular protein Tax1BP1 as a novel binding partner of human papillomavirus type 18 (HPV18) E2. Tax1BP1 also interacts with the HPV16 and bovine papillomavirus type 1 (BPV1) E2 proteins, with the C-terminal region of Tax1BP1 interacting with the N-terminal transactivation domain of BPV1 E2. Tax1BP1 complexes with p300 and acts synergistically as a coactivator with p300 to enhance E2-dependent transcription. Using chromatin immuno- precipitation assays, we show that Tax1BP1 and E2 localize to the long control region on the BPV1 genome. Tax1BP1 was recently reported to bind ubiquitin and to function as an essential component of an A20 ubiquitin-editing complex. We demonstrate that Tax1BP1 plays a role in the regulation of the steady-state level of E2 by preventing its proteasomal degradation. These studies provide new insights into the regulation of E2 functions.

UR - http://www.scopus.com/inward/record.url?scp=60049094359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60049094359&partnerID=8YFLogxK

U2 - 10.1128/JVI.01791-08

DO - 10.1128/JVI.01791-08

M3 - Article

C2 - 19109394

AN - SCOPUS:60049094359

VL - 83

SP - 2274

EP - 2284

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 5

ER -