TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells

Luciana I. Gallo, Yong Liao, Wily G. Ruiz, Dennis R. Clayton, Min Li, Yong Jian Liu, Yu Jiange, Mitsunori Fukuda, Gerard Apodaca, Xiao-Ming Yin

Research output: Contribution to journalArticle

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Abstract

Rab11a is a key modulator of vesicular trafficking processes, but there is limited information about the guanine nucleotide-exchange factors and GTPase-activating proteins (GAPs) that regulate its GTP-GDP cycle. We observed that in the presence of Mg2+ (2.5 mM), TBC1D9B interacted via its Tre2-Bub2-Cdc16 (TBC) domain with Rab11a, Rab11b, and Rab4a in a nucleotide-dependent manner. However, only Rab11a was a substrate for TBC1D9B-stimulated GTP hydrolysis. At limiting Mg2+ concentrations (<0.5 mM), Rab8a was an additional substrate for this GAP. In polarized Madin-Darby canine kidney cells, endogenous TBC1D9B colocalized with Rab11a-positive recycling endosomes but less so with EEA1-positive early endosomes, transferrin-positive recycling endosomes, or late endosomes. Overexpression of TBC1D9B, but not an inactive mutant, decreased the rate of basolateral-to-apical IgA transcytosis-a Rab11a-dependent pathway-and shRNA-mediated depletion of TBC1D9B increased the rate of this process. In contrast, TBC1D9B had no effect on two Rab11a-independent pathways-basolateral recycling of the transferrin receptor or degradation of the epidermal growth factor receptor. Finally, expression of TBC1D9B decreased the amount of active Rab11a in the cell and concomitantly disrupted the interaction between Rab11a and its effector, Sec15A. We conclude that TBC1D9B is a Rab11a GAP that regulates basolateral-to-apical transcytosis in polarized MDCK cells.

Original languageEnglish
Pages (from-to)3779-3797
Number of pages19
JournalMolecular Biology of the Cell
Volume25
Issue number23
DOIs
StatePublished - Nov 15 2014

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GTPase-Activating Proteins
Madin Darby Canine Kidney Cells
Endosomes
Transcytosis
Guanosine Triphosphate
Guanine Nucleotide Exchange Factors
Transferrin Receptors
Recycling
Transferrin
Epidermal Growth Factor Receptor
Immunoglobulin A
Small Interfering RNA
Hydrolysis
Nucleotides

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. / Gallo, Luciana I.; Liao, Yong; Ruiz, Wily G.; Clayton, Dennis R.; Li, Min; Liu, Yong Jian; Jiange, Yu; Fukuda, Mitsunori; Apodaca, Gerard; Yin, Xiao-Ming.

In: Molecular Biology of the Cell, Vol. 25, No. 23, 15.11.2014, p. 3779-3797.

Research output: Contribution to journalArticle

Gallo, LI, Liao, Y, Ruiz, WG, Clayton, DR, Li, M, Liu, YJ, Jiange, Y, Fukuda, M, Apodaca, G & Yin, X-M 2014, 'TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells', Molecular Biology of the Cell, vol. 25, no. 23, pp. 3779-3797. https://doi.org/10.1091/mbc.E13-10-0604
Gallo, Luciana I. ; Liao, Yong ; Ruiz, Wily G. ; Clayton, Dennis R. ; Li, Min ; Liu, Yong Jian ; Jiange, Yu ; Fukuda, Mitsunori ; Apodaca, Gerard ; Yin, Xiao-Ming. / TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. In: Molecular Biology of the Cell. 2014 ; Vol. 25, No. 23. pp. 3779-3797.
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AU - Gallo, Luciana I.

AU - Liao, Yong

AU - Ruiz, Wily G.

AU - Clayton, Dennis R.

AU - Li, Min

AU - Liu, Yong Jian

AU - Jiange, Yu

AU - Fukuda, Mitsunori

AU - Apodaca, Gerard

AU - Yin, Xiao-Ming

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