Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer

Hiroyuki Yamagishi, Jun Maeda, Tonghuan Hu, John McAnally, Simon Conway, Tsutomu Kume, Erik N. Meyers, Chihiro Yamagishi, Deepak Srivastava

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Haploinsufficiency of Tbx1 is likely a major determinant of cardiac and craniofacial birth defects associated with DiGeorge syndrome. Although mice deficient in Tbx1 exhibit pharyngeal and aortic arch defects, the developmental program and mechanisms through which Tbx1 functions are relatively unknown. We identified a single cis-element upstream of Tbx1 that recognized winged helix/forkhead box (Fox)-containing transcription factors and was essential for regulation of Tbx1 transcription in the pharyngeal endoderm and head mesenchyme. The Tbx1 regulatory region was responsive to signaling by Sonic hedgehog (Shh) in vivo. We show that Shh is necessary for aortic arch development, similar to Tbx1, and is also required for expression of Foxa2 and Foxc2 in the pharyngeal endoderm and head mesenchyme, respectively. Foxa2, Foxc1, or Foxc2 could bind and activate transcription through the critical cis-element upstream of Tbx1, and Foxc proteins were required, within their expression domains, for Tbx1 transcription in vivo. We propose that Tbx1 is a direct transcriptional target of Fox proteins and that Fox proteins may serve an intermediary role in Shh regulation of Tbx1.

Original languageEnglish (US)
Pages (from-to)269-281
Number of pages13
JournalGenes and Development
Volume17
Issue number2
DOIs
StatePublished - Jan 15 2003
Externally publishedYes

Fingerprint

Forkhead Transcription Factors
Hedgehogs
Endoderm
Mesoderm
Thoracic Aorta
Head
DiGeorge Syndrome
Branchial Region
Haploinsufficiency
Proteins
Nucleic Acid Regulatory Sequences

Keywords

  • DiGeorge syndrome
  • Fox
  • Shh
  • Tbx1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer. / Yamagishi, Hiroyuki; Maeda, Jun; Hu, Tonghuan; McAnally, John; Conway, Simon; Kume, Tsutomu; Meyers, Erik N.; Yamagishi, Chihiro; Srivastava, Deepak.

In: Genes and Development, Vol. 17, No. 2, 15.01.2003, p. 269-281.

Research output: Contribution to journalArticle

Yamagishi, H, Maeda, J, Hu, T, McAnally, J, Conway, S, Kume, T, Meyers, EN, Yamagishi, C & Srivastava, D 2003, 'Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer', Genes and Development, vol. 17, no. 2, pp. 269-281. https://doi.org/10.1101/gad.1048903
Yamagishi, Hiroyuki ; Maeda, Jun ; Hu, Tonghuan ; McAnally, John ; Conway, Simon ; Kume, Tsutomu ; Meyers, Erik N. ; Yamagishi, Chihiro ; Srivastava, Deepak. / Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer. In: Genes and Development. 2003 ; Vol. 17, No. 2. pp. 269-281.
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