Tea polyphenols down-regulate the expression of the androgen receptor in LNCaP prostate cancer cells

Fengge Ren, Shaobo Zhang, Susan H. Mitchell, Rachel Butler, Charles Y.F. Young

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Androgens via their cognate receptor may be involved in the development and progression of prostate cancer. The aim of this study was to determine whether tea polyphenols have inhibitory effects on androgen action in an androgen-responsive, prostate cancer cell line, LNCaP. The tea polyphenol, EGCG, inhibited LNCaP cell growth and the expression of androgen regulated PSA and hK2 genes. Moreover, EGCG had a significant inhibitory effect on the androgenic inducibility of the PSA promoter. Immunoblotting detected a decrease in androgen receptor protein with treatments of the tea polyphenols EGCG, GCG and theaflavins. Northern blot analysis showed decreased levels of androgen receptor mRNA by EGCG. Transient transfections demonstrated that EGCG and theaflavins could repress the transcriptional activities of the androgen receptor promoter region. An Sp1 binding site in the androgen receptor gene promoter is an important regulatory component for its expression. This study suggests Sp1 is the target for the tea polyphenols because treatments of EGCG decreased the expression, DNA binding activity and transactivation activity of Sp1 protein. In conclusion, we have described a new property of tea polyphenols that inhibits androgen action by repressing the transcription of the androgen receptor gene.

Original languageEnglish (US)
Pages (from-to)1924-1932
Number of pages9
JournalOncogene
Volume19
Issue number15
DOIs
StatePublished - Apr 6 2000
Externally publishedYes

Fingerprint

Androgen Receptors
Polyphenols
Tea
Androgens
Prostatic Neoplasms
Down-Regulation
Genes
Immunoblotting
Genetic Promoter Regions
Northern Blotting
Transcriptional Activation
Transfection
Proteins
Binding Sites
Cell Line
Messenger RNA
DNA
Growth
theaflavin

Keywords

  • Androgen receptor
  • Human glandular kallikrein
  • Prostate specific antigen
  • Sp1
  • Tea polyphenols
  • Theaflavins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Tea polyphenols down-regulate the expression of the androgen receptor in LNCaP prostate cancer cells. / Ren, Fengge; Zhang, Shaobo; Mitchell, Susan H.; Butler, Rachel; Young, Charles Y.F.

In: Oncogene, Vol. 19, No. 15, 06.04.2000, p. 1924-1932.

Research output: Contribution to journalArticle

Ren, Fengge ; Zhang, Shaobo ; Mitchell, Susan H. ; Butler, Rachel ; Young, Charles Y.F. / Tea polyphenols down-regulate the expression of the androgen receptor in LNCaP prostate cancer cells. In: Oncogene. 2000 ; Vol. 19, No. 15. pp. 1924-1932.
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