Tear sample collection using cellulose acetate absorbent filters

Marieh Esmaeelpour, Jun Cai, Patrick Watts, Mike Boulton, Paul J. Murphy

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To assess the use of cellulose acetate filter rods as a technique for tear collection. Method: The cellulose acetate rod (CR) was compared with the 'standard' glass capillary tube (CT), in a series of experiments, to assess: sample collection by collected volume size; the effect of tear stimulation on total tear protein concentration and major tear protein concentrations; and technique invasiveness. Results: No difference was found in concentrations for total protein, IgA (secretory immunoglobulin A), lactoferrin and lysozyme (p > 0.05) with no clinically significant increase in serum albumin to show serum leakage. Sample volume was higher for CR (p <0.005) and sample volume increased for stimulated collection with CR (p = 0.001). Dilution effect of a stimulated sample size was reliably shown only with CR (r = -0.66, p = 0.011). Using bovine albumin standard with CR and CT, a smaller sample volume (p <0.001) and a higher protein concentration (p <0.001) were extracted with CR. Conclusion: The cellulose rod offers a suitable alternative to the glass CT. It is able to quickly absorb a sample, allowing use for a wide range of sample sizes, while being minimally invasive.

Original languageEnglish (US)
Pages (from-to)577-583
Number of pages7
JournalOphthalmic and Physiological Optics
Volume28
Issue number6
DOIs
StatePublished - Nov 2008
Externally publishedYes

Fingerprint

Tears
Sample Size
Glass
Secretory Immunoglobulin A
Lactoferrin
Muramidase
acetylcellulose
Serum Albumin
Cellulose
Albumins
Proteins
Serum

Keywords

  • Cellulose rod
  • Glass capillary
  • Tear collection
  • Tear proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Optometry
  • Sensory Systems

Cite this

Tear sample collection using cellulose acetate absorbent filters. / Esmaeelpour, Marieh; Cai, Jun; Watts, Patrick; Boulton, Mike; Murphy, Paul J.

In: Ophthalmic and Physiological Optics, Vol. 28, No. 6, 11.2008, p. 577-583.

Research output: Contribution to journalArticle

Esmaeelpour, M, Cai, J, Watts, P, Boulton, M & Murphy, PJ 2008, 'Tear sample collection using cellulose acetate absorbent filters', Ophthalmic and Physiological Optics, vol. 28, no. 6, pp. 577-583. https://doi.org/10.1111/j.1475-1313.2008.00603.x
Esmaeelpour, Marieh ; Cai, Jun ; Watts, Patrick ; Boulton, Mike ; Murphy, Paul J. / Tear sample collection using cellulose acetate absorbent filters. In: Ophthalmic and Physiological Optics. 2008 ; Vol. 28, No. 6. pp. 577-583.
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