Technical principles and neurosurgical applications of fluorescein fluorescence using a microscope-integrated fluorescence module

Roberto Rey-Dios, Aaron A. Cohen-Gadol

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Background: Fluorescent technology has recently become a valuable tool in the surgical management of neoplastic and vascular lesions. The availability of microscope-integrated fluorescent modules has facilitated incorporation of this technology within the microsurgical workflow. The currently available microscope integrated modules use 5-aminolevulinic acid (5-ALA) and indocyanine green (ICG) as fluorophores. Methods: Fluorescein sodium is a fluorescent molecule that has been used specifically in ophthalmology for the treatment of retinal angiography. A new microscope-integrated fluorescent module has been recently developed for fluorescein. We employed this technology to maximize resection of tumors and perform intraoperative angiography to guide microsurgical management of aneurysms and arteriovenous malformations. Results: Fluorescein fluorescence allows the surgeon to appreciate fluorescent structures through the oculars while visualizing non-fluorescent tissues in near natural colors. Therefore, the operator can proceed with microsurgery under the fluorescent mode. We present three representative cases in which the use of fluorescein fluorescence was found useful in the surgeon's decision making during surgery. Conclusions: The applications of this new microscope-integrated fluorescent module are multiple, and include vascular and oncologic neurosurgery. Further clinical investigations with large patient cohorts are needed to fully establish the role of this new technology.

Original languageEnglish (US)
Pages (from-to)701-706
Number of pages6
JournalActa Neurochirurgica
Volume155
Issue number4
DOIs
StatePublished - Apr 1 2013

Keywords

  • Fluorescein
  • Fluorescence
  • Fluorescence microscopy
  • Neurosurgical procedures

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Fingerprint Dive into the research topics of 'Technical principles and neurosurgical applications of fluorescein fluorescence using a microscope-integrated fluorescence module'. Together they form a unique fingerprint.

  • Cite this