Techniques in Histomorphometry

Matthew R. Allen, David B. Burr

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Bone histomorphometry, the assessment of cellular and structural variables on histologic sections, is an essential technique for understanding tissue-level mechanisms of bone physiology. After obtaining the sample (biopsy in humans, tissue collection in animals), histologic specimen preparation involves several steps, including processing in plastic or paraffin, sectioning, and staining. Analysis of tissue sections can range from measures of overall structure to more detailed assessment of cellular activity. Dynamic histomorphometry, in which fluorochrome labels are administered in vivo, allows measurement of bone formation rates, a powerful outcome variable for understanding bone remodeling. Several diseases, including hyperparathyroidism, osteomalacia, osteoporosis, and Paget disease, as well as their treatments (both antiremodeling and anabolic therapies), have important histologic features that are useful for understanding tissue-level mechanisms.

Original languageEnglish (US)
Title of host publicationBasic and Applied Bone Biology
PublisherElsevier Inc.
Pages131-148
Number of pages18
ISBN (Print)9780124160156
DOIs
StatePublished - Aug 12 2013

Keywords

  • Bone biopsy
  • Bone histology
  • Dynamic histomorphometry
  • Fluorochrome labels

ASJC Scopus subject areas

  • Medicine(all)
  • Dentistry(all)

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    Allen, M. R., & Burr, D. B. (2013). Techniques in Histomorphometry. In Basic and Applied Bone Biology (pp. 131-148). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-416015-6.00007-1