Techniques to study nephron function

Microscopy and imaging

Bruce Molitoris, Ruben M. Sandoval

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recent advances in optics, computer sciences, fluorophores, and molecular techniques allow investigators the opportunity to study dynamic events within the functioning kidney with subcellular resolution. Investigators can now use two-photon microscopy to follow several complex heterogenous processes in organs such as the kidney with high spacial and temporal resolution. Repeat determinations over time within the same animal are possible and minimize animal use and interanimal variability. Furthermore, the ability to obtain volumetric data (3D) makes quantitative 4D (time) analysis possible. Finally, use of multiple fluorophores concurrently allows for three different or interactive processes to be observed simultaneously. Therefore, this approach compliments existing molecular, biochemical, and pharmacologic techniques by advancing in vivo data analysis and interpretation to subcellular levels for molecules without the requirement for fixation. Its use in the kidney is in its infancy but offers much promise for unraveling the complex interdependent physiologic and pathophysiologic processes known to contribute to cell function and disease.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Volume458
Issue number1
DOIs
StatePublished - May 2009

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Fluorophores
Nephrons
Microscopy
Microscopic examination
Animals
Kidney
Imaging techniques
Research Personnel
Computer science
Optics
Photons
Molecules

Keywords

  • Endocytosis
  • Imaging
  • Kidney
  • Membrane
  • Microscopy

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Techniques to study nephron function : Microscopy and imaging. / Molitoris, Bruce; Sandoval, Ruben M.

In: Pflugers Archiv European Journal of Physiology, Vol. 458, No. 1, 05.2009, p. 203-209.

Research output: Contribution to journalArticle

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