Telokin expression in A10 smooth muscle cells requires serum response factor

B. Paul Herring, Aiping Fan Smith

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Telokin transcription is initiated from a smooth muscle-specific promoter located in an intron of the smooth muscle myosin light chain kinase gene. We have previously identified a 310-base pair fragment of the promoter that mediates A10 smooth muscle cell-specific expression of telokin. In the current study, telokin-luciferase reporter gene assays in A10 cells and REF52 nonmuscle cells revealed that the promoter region between -81 and +80 contains the regulatory elements required to mediate the in vitro cell specificity of the promoter. Several positive-acting elements, including an E box, myocyte enhancer factor 2 (MEF2)-TATA box, and CArG-serum response element, were identified within this region. Telokin transcription in A10 smooth muscle cells requires all three transcription initiation sites and an AT-rich sequence between -71 and -62 that includes a TATA box. MEF2 interacts with the AT-rich region with low affinity; however, MEF2 binding is not required for transcriptional activity in A10 cells. Binding of serum response factor (SRF) to a CArG element proximal to the TATA sequence is also critical for high levels of transcription in A10 cells. Together these data suggest that an AT-rich motif, acting in concert with SRF and an unusual transcription initiation mechanism, is required for the cell-specific expression of the telokin promoter in A10 smooth muscle cells.

Original languageEnglish (US)
Pages (from-to)C1394-C1404
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number4 41-4
StatePublished - May 21 1997

Fingerprint

Serum Response Factor
antineoplaston A10
MEF2 Transcription Factors
Smooth Muscle Myocytes
Muscle
Transcription
Cells
AT Rich Sequence
TATA Box
Genes
Serum Response Element
Smooth Muscle Myosins
Myosin-Light-Chain Kinase
E-Box Elements
Transcription Initiation Site
Luciferases
Genetic Promoter Regions
Introns
Assays
Reporter Genes

Keywords

  • gene regulation
  • myosin light chain kinase
  • promoter

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Telokin expression in A10 smooth muscle cells requires serum response factor. / Herring, B. Paul; Smith, Aiping Fan.

In: American Journal of Physiology - Cell Physiology, Vol. 272, No. 4 41-4, 21.05.1997, p. C1394-C1404.

Research output: Contribution to journalArticle

Herring, B. Paul ; Smith, Aiping Fan. / Telokin expression in A10 smooth muscle cells requires serum response factor. In: American Journal of Physiology - Cell Physiology. 1997 ; Vol. 272, No. 4 41-4. pp. C1394-C1404.
@article{3ade272cc99c47229f1bdb56ef1fdc65,
title = "Telokin expression in A10 smooth muscle cells requires serum response factor",
abstract = "Telokin transcription is initiated from a smooth muscle-specific promoter located in an intron of the smooth muscle myosin light chain kinase gene. We have previously identified a 310-base pair fragment of the promoter that mediates A10 smooth muscle cell-specific expression of telokin. In the current study, telokin-luciferase reporter gene assays in A10 cells and REF52 nonmuscle cells revealed that the promoter region between -81 and +80 contains the regulatory elements required to mediate the in vitro cell specificity of the promoter. Several positive-acting elements, including an E box, myocyte enhancer factor 2 (MEF2)-TATA box, and CArG-serum response element, were identified within this region. Telokin transcription in A10 smooth muscle cells requires all three transcription initiation sites and an AT-rich sequence between -71 and -62 that includes a TATA box. MEF2 interacts with the AT-rich region with low affinity; however, MEF2 binding is not required for transcriptional activity in A10 cells. Binding of serum response factor (SRF) to a CArG element proximal to the TATA sequence is also critical for high levels of transcription in A10 cells. Together these data suggest that an AT-rich motif, acting in concert with SRF and an unusual transcription initiation mechanism, is required for the cell-specific expression of the telokin promoter in A10 smooth muscle cells.",
keywords = "gene regulation, myosin light chain kinase, promoter",
author = "Herring, {B. Paul} and Smith, {Aiping Fan}",
year = "1997",
month = "5",
day = "21",
language = "English (US)",
volume = "272",
pages = "C1394--C1404",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "4 41-4",

}

TY - JOUR

T1 - Telokin expression in A10 smooth muscle cells requires serum response factor

AU - Herring, B. Paul

AU - Smith, Aiping Fan

PY - 1997/5/21

Y1 - 1997/5/21

N2 - Telokin transcription is initiated from a smooth muscle-specific promoter located in an intron of the smooth muscle myosin light chain kinase gene. We have previously identified a 310-base pair fragment of the promoter that mediates A10 smooth muscle cell-specific expression of telokin. In the current study, telokin-luciferase reporter gene assays in A10 cells and REF52 nonmuscle cells revealed that the promoter region between -81 and +80 contains the regulatory elements required to mediate the in vitro cell specificity of the promoter. Several positive-acting elements, including an E box, myocyte enhancer factor 2 (MEF2)-TATA box, and CArG-serum response element, were identified within this region. Telokin transcription in A10 smooth muscle cells requires all three transcription initiation sites and an AT-rich sequence between -71 and -62 that includes a TATA box. MEF2 interacts with the AT-rich region with low affinity; however, MEF2 binding is not required for transcriptional activity in A10 cells. Binding of serum response factor (SRF) to a CArG element proximal to the TATA sequence is also critical for high levels of transcription in A10 cells. Together these data suggest that an AT-rich motif, acting in concert with SRF and an unusual transcription initiation mechanism, is required for the cell-specific expression of the telokin promoter in A10 smooth muscle cells.

AB - Telokin transcription is initiated from a smooth muscle-specific promoter located in an intron of the smooth muscle myosin light chain kinase gene. We have previously identified a 310-base pair fragment of the promoter that mediates A10 smooth muscle cell-specific expression of telokin. In the current study, telokin-luciferase reporter gene assays in A10 cells and REF52 nonmuscle cells revealed that the promoter region between -81 and +80 contains the regulatory elements required to mediate the in vitro cell specificity of the promoter. Several positive-acting elements, including an E box, myocyte enhancer factor 2 (MEF2)-TATA box, and CArG-serum response element, were identified within this region. Telokin transcription in A10 smooth muscle cells requires all three transcription initiation sites and an AT-rich sequence between -71 and -62 that includes a TATA box. MEF2 interacts with the AT-rich region with low affinity; however, MEF2 binding is not required for transcriptional activity in A10 cells. Binding of serum response factor (SRF) to a CArG element proximal to the TATA sequence is also critical for high levels of transcription in A10 cells. Together these data suggest that an AT-rich motif, acting in concert with SRF and an unusual transcription initiation mechanism, is required for the cell-specific expression of the telokin promoter in A10 smooth muscle cells.

KW - gene regulation

KW - myosin light chain kinase

KW - promoter

UR - http://www.scopus.com/inward/record.url?scp=0030938768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030938768&partnerID=8YFLogxK

M3 - Article

C2 - 9142867

AN - SCOPUS:0030938768

VL - 272

SP - C1394-C1404

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 4 41-4

ER -