Telokin expression is restricted to smooth muscle tissues during mouse development

B. Herring, Gary E. Lyons, April M. Hoggatt, Patricia Gallagher

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Telokin is a 17-kDa protein with an amino acid sequence that is identical to the COOH terminus of the 130-kDa myosin light chain kinase (MLCK). Telokin mRNA is transcribed from a second promoter, located within an intron, in the 3′ region of the MLCK gene. In the current study, we show by in situ mRNA hybridization that telokin mRNA is restricted to the smooth muscle cell layers within adult smooth muscle tissues. In situ mRNA analysis of mouse embryos also revealed that telokin expression is restricted to smooth muscle tissues during embryonic development. Telokin mRNA expression was first detected in mouse gut at embryonic day 11.5; no telokin expression was detected in embryonic cardiac or skeletal muscle. Expression of telokin was also found to be regulated during postnatal development of the male and female reproductive tracts. In both uterus and vas deferens, telokin protein expression greatly increased between days 7 and 14 of postnatal development. The increase in telokin expression correlated with an increase in the expression of several other smooth muscle-restricted proteins, including smooth muscle myosin and α-actin.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume280
Issue number1 49-1
StatePublished - 2001

Fingerprint

Smooth Muscle
Muscle
Tissue
Muscles
Messenger RNA
Myosin-Light-Chain Kinase
Smooth Muscle Myosins
telokin
Proteins
Vas Deferens
Muscle Proteins
Introns
Uterus
Smooth Muscle Myocytes
Embryonic Development
In Situ Hybridization
Actins
Amino Acid Sequence
Myocardium
Skeletal Muscle

Keywords

  • Embryos
  • In situ hybridization
  • Myosin light chain kinase
  • Reproductive tract development

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Telokin expression is restricted to smooth muscle tissues during mouse development. / Herring, B.; Lyons, Gary E.; Hoggatt, April M.; Gallagher, Patricia.

In: American Journal of Physiology - Cell Physiology, Vol. 280, No. 1 49-1, 2001.

Research output: Contribution to journalArticle

Herring, B. ; Lyons, Gary E. ; Hoggatt, April M. ; Gallagher, Patricia. / Telokin expression is restricted to smooth muscle tissues during mouse development. In: American Journal of Physiology - Cell Physiology. 2001 ; Vol. 280, No. 1 49-1.
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