Temporal and spatial appearance of α-dystroglycan in differentiated mouse myoblasts in culture

Tatiana Kostrominova, M. L. Tanzer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The dystrophin-glycoprotein complex plays an important role in muscle function. One of the components of the complex, a 156-kDa cell surface glycoprotein (α-dystroglycan) binds to laminin, thereby connecting the basal lamina and muscle cells. We have examined the progressive appearance of α- dystroglycan and laminin in muscle cells that differentiate in culture. We find that nondifferentiated cultures of C2C12 myoblasts express low amounts of dystroglycan mRNA and, in contrast, this gene is prominently expressed in differentiated myotubes. Immunofluorescence analysis with a monoclonal antibody against α-dystroglycan shows its progressive appearance during myoblast differentiation into myotubes. Immunostaining with a monoclonal antibody against laminin shows that it is not present on the surface of undifferentiated myoblasts. Subsequently, laminin becomes apparent on the surface of differentiated myotubes where it codistributes with immunostained α-dystroglycan. Immunoblotting of isolated surface membrane proteins of differentiated myotubes with antibodies against α-dystroglycan identifies a broad band of about 140-160 kDa, resembling α-dystroglycan from rabbit muscle. The composite results indicate that α-dystroglycan and laminin appear and become co-distributed on the surface of cultured C2C12 during the progression of differentiation.

Original languageEnglish (US)
Pages (from-to)527-534
Number of pages8
JournalJournal of Cellular Biochemistry
Volume58
Issue number4
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Dystroglycans
Myoblasts
Laminin
Skeletal Muscle Fibers
Muscle
Muscle Cells
Membrane Proteins
Monoclonal Antibodies
Cells
Muscles
Dystrophin
Membrane Glycoproteins
Cell culture
Immunoblotting
Basement Membrane
Fluorescent Antibody Technique
Glycoproteins
Genes
Rabbits
Messenger RNA

Keywords

  • α-dystroglycan
  • differentiation
  • laminin
  • myoblasts

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Temporal and spatial appearance of α-dystroglycan in differentiated mouse myoblasts in culture. / Kostrominova, Tatiana; Tanzer, M. L.

In: Journal of Cellular Biochemistry, Vol. 58, No. 4, 1995, p. 527-534.

Research output: Contribution to journalArticle

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